Impact of the rift valley on restriction fragment length polymorphism typing of the major African malaria vector Anopheles funestus (Diptera: Culicidae)
Journal of Medical Entomology
Vector Control Reference Unit, National Institute for Communicable Diseases, Private bag X 4, Sandringham, 2131, South Africa; Division of Virology and Communicable Disease Surveillance, School of Pathology of the National Health Laboratory Service, University of the Witwatersrand, Johannesburg, South Africa; Centre for Biotechnology Research and Development, Kenya Medical Research Institute, P.O. Box 54840, Nairobi 00200, Kenya; Institut de Recherche Pour le Développement (IRD), UMR Centre de Biologie et de Gestion des Populations, Montpellier, France; School of Animal, Plant and Environmental Sciences, University of Witwatersrand, Private bag X 3, Wits 2080, South Africa
Anopheles funestus Giles (Diptera: Culicidae) is one of Africa's major malaria vectors. To understand population structure within An. funestus, various molecular markers have recently been developed. We investigated the impact of the Rift Valley on one such molecular marker, a polymerase chain reaction-restriction fragment length polymorphism (RFLP). This system distinguishes An. funestus populations into types M, W, and MW. Generally, these types correlate between geographical location and RFLP type, whereby M associates essentially with eastern Africa, W with western and central Africa, and MW with southern Africa. In total, 606 specimens were screened from 12 countries. Specimens from southern African localities were of the expected MW-type with the exception of the sample from Malawi, which showed all three types. The specimens from Tanzania showed the M- and MW-types, whereas specimens from Kenya showed M- and W-types. We also recorded different RFLP types not recorded previously. Type Y was recorded in Malawi, and Z-type was recorded from four localities: Angola, Malawi, Ghana, and Zambia. © 2006 Entomological Society of America.
Africa; animal; Anopheles; article; classification; comparative study; demography; disease carrier; DNA sequence; genetics; geography; molecular genetics; nucleotide sequence; parasitology; Plasmodium; population genetics; restriction fragment length polymorphism; Africa, Eastern; Animals; Anopheles; Base Sequence; Demography; Genetics, Population; Geography; Insect Vectors; Molecular Sequence Data; Plasmodium; Polymorphism, Restriction Fragment Length; Sequence Analysis, DNA; Anopheles funestus; Culicidae; Diptera