Umeyor E.C., Kenechukwu F.C., Ogbonna J.D., Chime S.A., Attama A.
School of Pharmacy, University of Nigeria, Nsukka 410001, Nigeria
Umeyor, E.C., School of Pharmacy, University of Nigeria, Nsukka 410001, Nigeria; Kenechukwu, F.C., School of Pharmacy, University of Nigeria, Nsukka 410001, Nigeria; Ogbonna, J.D., School of Pharmacy, University of Nigeria, Nsukka 410001, Nigeria; Chime, S.A., School of Pharmacy, University of Nigeria, Nsukka 410001, Nigeria; Attama, A., School of Pharmacy, University of Nigeria, Nsukka 410001, Nigeria
Objective: To formulate and evaluate solid-reversed-micellar-solution (SRMS)-based solid lipid microparticles (SLMs) for intramuscular administration of gentamicin. Methods: SRMS formulated with Phospholipon® 90G and Softisan® 154 were used to prepare gentamicin-loaded SLMs. Characterizations based on size and morphology, stability and encapsulation efficiency (EE%) were carried out on the SLMs. In vitro release of gentamicin from the SLMs was performed in phosphate buffer while in vivo release studies were conducted in rats. Results: Maximum EE% of 90.0, 91.6 and 83.0% were obtained for SLMs formed with SRMS 1:1, 1:2 and 2:1, respectively. Stable, spherical and smooth SLMs of size range 9.80±1.46 μm to 33.30±6.42 μm were produced. The release of gentamicin in phosphate buffer varied widely with the lipid contents. Moreover, significant (p<0.05) amount of gentamicin was released in vivo from the SLMs. Conclusion: SRMS-based SLMs would likely offer a reliable means of delivering gentamicin intramuscularly. © 2012 Informa UK Ltd. All rights reserved.
gentamicin; phosphate buffered saline; phosphatidylcholine; solid lipid nanoparticle; animal experiment; antibiotic sensitivity; article; chemical structure; controlled study; drug formulation; drug release; drug screening; drug stability; Escherichia coli; in vitro study; in vivo study; male; micelle; microencapsulation; nonhuman; particle size; rat; Staphylococcus aureus; Animals; Buffers; Calorimetry, Differential Scanning; Chemistry, Pharmaceutical; Drug Carriers; Drug Compounding; Drug Delivery Systems; Gentamicins; Hydrogen-Ion Concentration; Injections, Intramuscular; Kinetics; Lipids; Micelles; Microspheres; Particle Size; Phosphates; Rats; Temperature