Kiravu A., Gumbi P., Mkhize N.N., Olivier A., Denny L., Passmore J.-A.
Institute of Infectious Disease and Molecular Medicine, University of Cape Town Observatory, Cape Town, South Africa; Dept Obstetrics and Gynaecology, University of Cape Town and Groote Schuur Hospital, Cape Town, South Africa; National Health Laboratory Services, Cape Town, South Africa
Kiravu, A., Institute of Infectious Disease and Molecular Medicine, University of Cape Town Observatory, Cape Town, South Africa; Gumbi, P., Institute of Infectious Disease and Molecular Medicine, University of Cape Town Observatory, Cape Town, South Africa; Mkhize, N.N., Institute of Infectious Disease and Molecular Medicine, University of Cape Town Observatory, Cape Town, South Africa; Olivier, A., Institute of Infectious Disease and Molecular Medicine, University of Cape Town Observatory, Cape Town, South Africa; Denny, L., Dept Obstetrics and Gynaecology, University of Cape Town and Groote Schuur Hospital, Cape Town, South Africa; Passmore, J.-A., Institute of Infectious Disease and Molecular Medicine, University of Cape Town Observatory, Cape Town, South Africa, National Health Laboratory Services, Cape Town, South Africa
Mucosal homing receptors expressed by blood T cells may be useful surrogates for measuring mucosal T cell immune responses at the site of HIV transmission. Here, we investigated whether HIV-specific responses by T cells expressing the mucosal integrin receptor CD103 in blood reliably predicted corresponding HIV-specific responses at the cervix. The frequency of CD8+ T cells expressing CD103 in blood correlated significantly with the number of CD103. +. CD8+ T cells from the cervix suggesting that CD103 was involved in trafficking of T cells from blood to the cervical mucosa. TGF-β concentrations in plasma were significantly associated with the frequency of CD103 expression by blood but not cervical CD8 T cells. The majority of Gag-responsive CD8 T cells were CD103+ in both blood and at the cervix. Despite this, the magnitude of Gag-specific IFN-γ responses by CD103. +. CD8+ T cells in blood did not predict similar Gag-specific responses at the cervix. © 2011 Elsevier Inc.
CD103 antigen; Gag protein; gamma interferon; interleukin 10; interleukin 1beta; interleukin 8; transforming growth factor beta; tumor necrosis factor alpha; adult; antigen expression; article; CD8+ T lymphocyte; cell migration; cellular immunity; clinical article; controlled study; cytokine production; disease marker; female; human; human cell; Human immunodeficiency virus infection; lymphocyte count; priority journal; uterine cervix; uterine cervix mucosa; virus load; Adult; Antigens, CD; Biological Markers; Body Fluids; CD8-Positive T-Lymphocytes; Cervix Uteri; Chemotaxis, Leukocyte; Cytokines; Female; Gene Expression Regulation; Gene Products, gag; HIV Infections; HIV-1; Humans; Immunity, Mucosal; Immunologic Memory; Integrin alpha Chains; Interferon-gamma; Organ Specificity; T-Lymphocyte Subsets; Transforming Growth Factor beta1; Viral Load