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6033

Source

Scopus

Electronic ID

2-s2.0-77649106795

Authors

Matemadombo F., Durmus M., Escriou V., Griveau S., Scherman D., Bedioui F., Nyokong T.

Title

Evaluation of the performance of manganese phthalocyanines as superoxide dismutase mimics

Publication Year

2009

Source Title

Current Analytical Chemistry

Volume

Issue

Citations

DOI

10.2174/157341109789077731

URL

https://www.scopus.com/inward/record.uri?eid=2-s2.0-77649106795&partnerID=40&md5=b45d2ee88a7c69099f1548d49f1759bd

Affiliations

INSERM, U640, Paris, F-75006, France; CNRS, UMR8151, Paris, F-75006, France; Université Paris Descartes, Faculté de Pharmacie, Unité de Pharmacologie Chimique et Génétique, Paris, F-75270, France; Ecole Nationale Supérieure de Chimie de Paris, Paris, F-75005, France; Rhodes University, Department of Chemistry, P.O. Box 94, 6140, Grahamstown, South Africa; Gebze Institute of Technology, Department of Chemistry, P.O. Box: 141, 41400, Gebze-Kocaeli, Turkey

Authors with Affiliations

Matemadombo, F., INSERM, U640, Paris, F-75006, France, CNRS, UMR8151, Paris, F-75006, France, Université Paris Descartes, Faculté de Pharmacie, Unité de Pharmacologie Chimique et Génétique, Paris, F-75270, France, Ecole Nationale Supérieure de Chimie de Paris, Paris, F-75005, France, Rhodes University, Department of Chemistry, P.O. Box 94, 6140, Grahamstown, South Africa; Durmus, M., Gebze Institute of Technology, Department of Chemistry, P.O. Box: 141, 41400, Gebze-Kocaeli, Turkey; Escriou, V., INSERM, U640, Paris, F-75006, France, CNRS, UMR8151, Paris, F-75006, France, Université Paris Descartes, Faculté de Pharmacie, Unité de Pharmacologie Chimique et Génétique, Paris, F-75270, France, Ecole Nationale Supérieure de Chimie de Paris, Paris, F-75005, France, Rhodes University, Department of Chemistry, P.O. Box 94, 6140, Grahamstown, South Africa; Griveau, S., INSERM, U640, Paris, F-75006, France, CNRS, UMR8151, Paris, F-75006, France, Université Paris Descartes, Faculté de Pharmacie, Unité de Pharmacologie Chimique et Génétique, Paris, F-75270, France, Ecole Nationale Supérieure de Chimie de Paris, Paris, F-75005, France, Rhodes University, Department of Chemistry, P.O. Box 94, 6140, Grahamstown, South Africa; Scherman, D., INSERM, U640, Paris, F-75006, France, CNRS, UMR8151, Paris, F-75006, France, Université Paris Descartes, Faculté de Pharmacie, Unité de Pharmacologie Chimique et Génétique, Paris, F-75270, France, Ecole Nationale Supérieure de Chimie de Paris, Paris, F-75005, France, Rhodes University, Department of Chemistry, P.O. Box 94, 6140, Grahamstown, South Africa; Bedioui, F., INSERM, U640, Paris, F-75006, France, CNRS, UMR8151, Paris, F-75006, France, Université Paris Descartes, Faculté de Pharmacie, Unité de Pharmacologie Chimique et Génétique, Paris, F-75270, France, Ecole Nationale Supérieure de Chimie de Paris, Paris, F-75005, France, Rhodes University, Department of Chemistry, P.O. Box 94, 6140, Grahamstown, South Africa; Nyokong, T., Rhodes University, Department of Chemistry, P.O. Box 94, 6140, Grahamstown, South Africa

Abstract

Evaluation of the performance of seven variously substituted manganese(III) phthalocyanine complexes (MnPcs) for the dismutation of superoxide radical (O2 •-), as superoxide dismutase mimics (SOD mimics), was assessed using cyclic voltammetry, UV visible spectrophotometry and fluorescence. In a first step, the electrochemical analysis of the MnPcs allows showing the fine tuning of the redox potential of the MnIII/MnII couple which is involved in the dismutation process of O2 •-. Thus, the evaluation of the behaviour of the MPcs as SOD mimics were tested toward O2 •- produced from the xanthine-xanthine oxidase reaction, using cytochrome c and UV visible spectrophometry (McCord-Fridovich assay). All manganese phthalocyanine complexes did not interfere with the xanthine-xanthine oxidase reaction and efficiently dismutated O2 •- with apparent overall catalytic rate constant values ranging from log kcat = 7.81 to 6.62 and improved IC50 (concentration of complex that induces 50% inhibition of the reduction of 10μM cytochrome c) values (0.04 to 0.68 μM) compared to one commercially available Mn porphyrin based SOD mimic (namely manganese (III) tetrakis (1-methyl-4-pyridyl)porphyrin). We used DMSO-treated HL60 cell (human leukemia cells) as a model of O2 •- production, either extracellular production with Phorbol 12-myristate 13-acetate (PMA) stimulation or intracellular production without PMA stimulation. With these models, analysis of dihydroethidium (DHE) fluorescence for the detection of superoxide production showed that the examined MnPcs act as proficient superoxide dismutase mimics. The obtained results also showed that four of these MPcs are cell permeant. © 2009 Bentham Science Publishers Ltd.

Author Keywords

Cyclic voltammetry; Fluorescence; HL-60; Manganese phthalocyanine; SOD mimic; Superoxide radical; UV visible spectrophotometry

Index Keywords

None

Funding Details

None