Department of Veterinary Surgery and Reproduction, Faculty of Veterinary Medicine, University of Ibadan, Nigeria
Olqyemi, F.O., Department of Veterinary Surgery and Reproduction, Faculty of Veterinary Medicine, University of Ibadan, Nigeria; Adeniji, D.A., Department of Veterinary Surgery and Reproduction, Faculty of Veterinary Medicine, University of Ibadan, Nigeria; Oyeyemi, M.O., Department of Veterinary Surgery and Reproduction, Faculty of Veterinary Medicine, University of Ibadan, Nigeria
The proportion of honey, egg yolk and sodium citrate buffer suitable for the extension of West African Dwarf (WAD) buck (Capra hircus L.) spermatozoa at 5°C was studied. Semen was collected from four clinically healthy bucks certified free of any obvious andrological defects. Four diluents were prepared with egg yolk citrate as the control while other diluents consisted of increasing concentrations of honey and decreasing concentrations of egg yolk. Evaluations for motility and viability were done every two hours for six hours for each diluent. Results show that inclusion of honey in egg yolk based extender sustained sperm motility and liveability for up to 6 hours at 5°C. This effect was however found to be dependent on the ratio of honey to egg yolk in the extender. Diluent 2 (5ml honey + 15ml egg yolk + 80ml sodium citrate) gave the highest percentage motility at each of the hours tested. The live dead ratio observed at different hours gave high values for diluents 1, 2 and 3 with diluent 2 as the highest. Diluent 4 (20ml honey + 80ml sodium citrate) gave very low values except at 0 hour.The result of this study suggested that the addition of honey to egg yolk improves the motility and live dead ratio and thus viability of liquid goat semen. However, this effect is concentration dependent and higher concentrations may have negative effect on sperm viability. © IDOSI Publications, 2011.
