Department of Pharmacy, College of Health Science, Mekelle University, P.O. Box 1871, Mekelle, Ethiopia; Department of Pharmacology and Clinical Pharmacy, School of Pharmacy, Addis Ababa University, Addis Ababa, Ethiopia
Kebebew, Z., Department of Pharmacy, College of Health Science, Mekelle University, P.O. Box 1871, Mekelle, Ethiopia; Shibeshi, W., Department of Pharmacology and Clinical Pharmacy, School of Pharmacy, Addis Ababa University, Addis Ababa, Ethiopia
Ethnopharmacological relevance: Carica papaya has been used in the Ethiopian traditional medicine to relieve stress and other disease conditions. Aim of the study: The present study was undertaken to evaluate the anxiolytic and sedative effects of 80% ethanolic Carica papaya (Caricaceae) pulp extract in mice. Materials and methods Carica papaya pulp extract was screened for anxiolytic effect by using elevated plus maze, staircase and open field tests, and ketamine-induced sleeping time test for sedation at doses of 50, 100, 200, 400 mg/kg. Distilled water and Diazepam were employed as negative and positive control groups, respectively. Results: Carica papaya pulp extract 100 mg/kg significantly increased the percentage of open arm time and entry, and reduced the percentage of entry and time spent in closed arm in elevated plus maze test; reduced the number of rearing in the staircase test; and increased the time spent and entries in the central squares while the total number of entries into the open field were not significantly affected, suggesting anxiolytic activity without altering locomotor and sedative effects. A synergistic reduction in the number of rearing and an inverted U-shaped dose response curves were obtained with important parameters of anxiety Conclusions: The results of this study established a support for the traditional usage of Carica papaya as anxiolytic medicinal plant. © 2013 Elsevier Ireland Ltd. All rights reserved.
alcohol; alkaloid derivative; anxiolytic agent; Carica papaya extract; diazepam; flavonoid; ketamine; phenol derivative; phlobatannin derivative; saponin derivative; sedative agent; tannin derivative; terpenoid derivative; unclassified drug; animal experiment; anxiety; article; controlled study; dose response; drug efficacy; drug isolation; drug potentiation; drug screening; elevated plus maze test; experimental test; female; male; maze test; mouse; nonhuman; open field test; papaya; phytochemistry; rearing; sedation; sleep time; staircase test; tranquilizing activity; Carica papaya; Caricaceae; Mus; Anxiolytic; Carica papaya; Mice; Sedative; Animals; Anti-Anxiety Agents; Anxiety; Behavior, Animal; Carica; Ethanol; Exploratory Behavior; Female; Fruit; Hypnotics and Sedatives; Lethal Dose 50; Male; Mice; Plant Extracts; Solvents; Toxicity Tests, Acute
