Phytochemical and anti inflammatory evaluation of Khaya grandifoliola stem bark extract
International Journal of PharmTech Research
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Benin, Benin City, Nigeria; Department of Pharmacology, College of Health Sciences, Niger Delta University, Wilberforce Island, Yenagoa, Nigeria
The ethnomedicinal application of Khaya grandifoliola stem bark in Africa as remedy against rheumatoid arthritis is attracting attention in view of the global focus on herbal medicine as alternatives to orthodox drugs. And this necessitated this study.The methanolic extract of the plant was subjected to phytochemical screening testing for alkaloids, tannins, saponins and flavonoids as secondary metabolites. Anti inflammatory activity of the methanolic extract was examined using carrageenan, and serotonin -induced, paw oedema and xylene induced ear oedema. The methanolic extract significantly inhibited the carrageenan-induced paw oedema from the first hour to the fifth hour at a dose of 200mg/kg while a dose of 500 mg/kg significantly inhibited the carrageenan-induce paw oedema after 3 hours of carrageenan challenge. Whereas in the serotonin induced paw oedema test, the extract significantly reduces the paw oedema at the 120th minute at the dose of 200 mg/kg and at 90th minute at the dose of 500 mg/kg. For the xylene induced oedema there was a 28.6 % inhibition at 200 mg/kg and 57.1% at 500 mg/kg. The result of the study revealed that the methanolic extract of K.grandifoliola was active at different doses tested. This gives justification to the use of the plant as anti inflammatory medicinal plant.
alkaloid; carrageenan; dexamethasone; indometacin; Khaya grandifoliola extract; methanol; plant extract; saponin; serotonin; tannin; unclassified drug; xylene; Africa; animal experiment; animal model; antiinflammatory activity; article; bark; controlled study; drug dose comparison; Khaya grandifoliola; medicinal plant; nonhuman; otitis; paw edema; phytochemistry; plant stem; rat; time; traditional medicine; treatment outcome