Evaluation of antibacterial activity of Piliostigma thonningiin (crude extract) and fractions 1-3, 4, 5, 6 and 7 against Methicillin-resistant staphylococcus aureus (MRSA)
Research Journal of Pharmaceutical, Biological and Chemical Sciences
Department of Microbiology, Federal Univerity of Technology, P.M.B. 65, Minna, Nigeria; Department of Biochemistry, Federal Univerity of Technology, P.M.B. 65, Minna, Nigeria; Department of Microbiology, Kogi State University, Ayigba, Nigeria; Department
Aim of this study is to investigate the antibacterial activity of traditional Nigeria medicines with special interest in anti-methicillin-resistant Staphylococcus aureus (MRSA) activity. Hexane, ethylacetate, methanol and aqueous extracts of this plant was prepared. Agar dilution, agar-well diffusion tests and MIC's were used to investigate antibacterial activity. Out of 4 extract that were evaluated for antibacterial activity using agar dilution technique only hexane extract gave the highest inhibition against all the MRSA isolates. Crude extract and fractions showed the highest levels of antibacterial activity overall with MIC's against MRSA in the range of 1.0 and 2.0mg/ml. The presence of antibacterial activity in extracts and fractions of leaves of P. thonningii plants traditionally used to treat skin infections, are reported for the first time. Extracts from P. thonningii, also used to treat wounds, had the widest spectrum of antibacterial activity.
Piliostigma thonningiin extract; plant extract; unclassified drug; vancomycin; agar dilution; antibacterial activity; article; bacterium isolate; diffusion; drug isolation; medicinal plant; methicillin resistant Staphylococcus aureus; minimum inhibitory concentration; Nigeria; nonhuman; Piliostigma thonningiin; plant leaf; methicillin resistant Staphylococcus aureus; Piliostigma; Piliostigma thonningii