Ogunlade I., Tucker G., Fisk I., Ogunlade A.
Department of Chemistry, University of Ado-Ekiti, Ekiti State, Nigeria; Division of Nutritional Sciences, Division of Food Science, Nigeria; School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, United Kingdom; School of Physiology, Nutrition and Consumer Sciences, North-West University (Potchefstroom Campus), South Africa
Ogunlade, I., Department of Chemistry, University of Ado-Ekiti, Ekiti State, Nigeria, Division of Nutritional Sciences, Division of Food Science, Nigeria; Tucker, G., Division of Nutritional Sciences, Division of Food Science, Nigeria; Fisk, I., School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, United Kingdom; Ogunlade, A., School of Physiology, Nutrition and Consumer Sciences, North-West University (Potchefstroom Campus), South Africa
Freeze-dried leaves of some selected vegetables (Cnidoscolus aconitifolius, Talinum fruticosum and Solanum macrocarpon) were examined for antioxidant activity and analysed for vitamin E profile, using Ferric-ion Reducing Antioxidant Power (FRAP) assay. Total phenolic content (TPC) was measured by Folin-Ciocalteu method, and vitamin E profile was determined by HPLC analysis. Based on dry weight of the vegetables, C. aconitifolius had the highest antioxidant activity (122 μ mole Trolox equivalent/g dry wt), followed by S. macrocarpon (115.2 μmole TE/g dry wt) and T. fruticosum (78.14 μmole TE/g dry wt). Total phenolic content ranged from 349.2 to 1247.6 mg GAE/g dry wt for T. fruticosum, S. macrocarpon and C. aconitifolius, respectively. Vitamin E profile (tocopherol & tocotrienols) was significantly higher in C. aconitifolius (p<0.05) than in other two vegetables. The results suggest that these vegetables when consumed can be utilized to block/scavenge free radicals thereby alleviating symptoms associated with chronic and degenerative diseases.
alpha tocopherol; alpha tocotrienol; antioxidant; ferric ion; free radical; phenol; tocopherol; antioxidant activity; article; Cnidoscolus aconitifolius; dry weight; evaluation; fluorescence recovery after photobleaching; high performance liquid chromatography; Nigeria; nonhuman; Solanum macrocarpon; Talinum fruticosum; vegetable; Cnidoscolus aconitifolius; Solanum macrocarpon; Talinum fruticosum
