Evaluation of a direct, rapid immunohistochemical test for rabies diagnosis
Emerging Infectious Diseases
University of Edinburgh, Midlothian, United Kingdom; Centers for Disease Control and Prevention, Atlanta, GA, United States; Tanzania Wildlife Research Institute, Arusha, Tanzania; Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, United States
A direct rapid immunohistochemical test (dRIT) was evaluated under field and laboratory conditions to detect rabies virus antigen in frozen and glycerol-preserved field brain samples from northwestern Tanzania. Compared to the direct fluorescent antibody test, the traditional standard in rabies diagnosis, the dRIT was 100% sensitive and specific.
virus antigen; animal tissue; article; brain tissue; controlled study; diagnostic test; evaluation; field study; fluorescent antibody technique; frozen section; immunohistochemistry; intermethod comparison; laboratory test; nonhuman; rabies; Rabies virus; sensitivity and specificity; Tanzania; tissue preservation; Animals; Animals, Domestic; Animals, Wild; Antigens, Viral; Brain; Cats; Cattle; Dogs; Glycerol; Immunohistochemistry; Population Surveillance; Rabies; Rabies virus; Sensitivity and Specificity; Tanzania; Time Factors; Tissue Preservation