Maturation and Mip-1β production of cytomegalovirus-specific T cell responses in Tanzanian children, adolescents and adults: Impact by HIV and Mycobacterium tuberculosis co-infections
Swiss Tropical and Public Health Institute, Basel, Switzerland; University of Basel, Basel, Switzerland; NIMR-Mbeya Medical Research Centre, Mbeya, Tanzania; Department of Infectious Diseases and Tropical Medicine, Medical Centre of the University of Munich, Munich, Germany; Ifakara Health Institute, Bagamoyo, Tanzania; German Centre for Infection Research (DZIF), partner site Munich, Munich, Germany
It is well accepted that aging and HIV infection are associated with quantitative and functional changes of CMV-specific T cell responses. We studied here the expression of Mip-1β and the T cell maturation marker CD27 within CMVpp65-specific CD4+ and CD8+ T cells in relation to age, HIV and active Tuberculosis (TB) co-infection in a cohort of Tanzanian volunteers (≤16 years of age, n = 108 and ≥18 years, n = 79). Independent of HIV co-infection, IFNγ+ CMVpp65-specific CD4+ T cell frequencies increased with age. In adults, HIV co-infection further increased the frequencies of these cells. A high capacity for Mip-1β production together with a CD27low phenotype was characteristic for these cells in children and adults. Interestingly, in addition to HIV co-infection active TB disease was linked to further down regulation of CD27 and increased capacity of Mip-1β production in CMVpp65-specific CD4+ T cells. These phenotypic and functional changes of CMVpp65-specific CD4 T cells observed during HIV infection and active TB could be associated with increased CMV reactivation rates. © 2015 Portevin et al.
CD27 antigen; macrophage inflammatory protein 1beta; adolescent; adult; age; Article; CD4+ T lymphocyte; CD8+ T lymphocyte; child; controlled study; Cytomegalovirus; down regulation; human; Human immunodeficiency virus infection; lymphocyte subpopulation; major clinical study; mixed infection; nonhuman; phenotype; protein expression; protein processing; protein synthesis; T lymphocyte; Tanzanian; tuberculosis; virus reactivation; Cytomegalovirus; Mycobacterium tuberculosis