Gari G., Biteau-Coroller F., LeGoff C., Caufour P., Roger F.
Sebeta National Animal Health Research Center, PO Box 04, Sebeta, Ethiopia; CIRAD, Epidemiology and Ecology, Animal Diseases Unit, TA A-16/E, Campus International Baillarguet, 34398 Montpellier Cedex 5, France; CIRAD, Emerging and Exotic Animal Disease Control Unit, TA A-15/G, Campus International Baillarguet, 34398 Montpellier Cedex 5, France
Gari, G., Sebeta National Animal Health Research Center, PO Box 04, Sebeta, Ethiopia, CIRAD, Epidemiology and Ecology, Animal Diseases Unit, TA A-16/E, Campus International Baillarguet, 34398 Montpellier Cedex 5, France; Biteau-Coroller, F., CIRAD, Epidemiology and Ecology, Animal Diseases Unit, TA A-16/E, Campus International Baillarguet, 34398 Montpellier Cedex 5, France; LeGoff, C., CIRAD, Emerging and Exotic Animal Disease Control Unit, TA A-15/G, Campus International Baillarguet, 34398 Montpellier Cedex 5, France; Caufour, P., CIRAD, Emerging and Exotic Animal Disease Control Unit, TA A-15/G, Campus International Baillarguet, 34398 Montpellier Cedex 5, France; Roger, F., CIRAD, Epidemiology and Ecology, Animal Diseases Unit, TA A-16/E, Campus International Baillarguet, 34398 Montpellier Cedex 5, France
The performance of indirect fluorescence antibody test (IFAT) for serological diagnosis and screening of lumpy skin disease (LSD) was evaluated using methods without gold standard. Virus neutralization test (VNT) was used as the second test and the study sites were selected from two different geographical places in Ethiopia to get different disease prevalence. The analysis of conditional dependent Bayesian model for the accuracy of IFAT showed that sensitivity, specificity, prevalence of the population Pi1 and the population Pi2 were 0.92 (0.89-0.95), 0.88 (0.85-0.91), 0.28 (0.25-0.32) and 0.06 (0.048-0.075), respectively. The posterior inferences obtained for VNT sensitivity, specificity and conditional correlation between the tests for sensitivity (rhoD) and specificity (rhoDc) were 0.78 (0.74-0.83), 0.97 (0.95-0.99), 0.052 (-0.03-0.15) and 0.019 (-0.01-0.06), respectively. The interval estimation of conditional correlation for both sensitivity and specificity clusters around zero and thus conditional dependence between the two tests was not significant. Although accuracy measure would not be the only basis for test selection, the result of our study demonstrated that IFAT has a reasonable high accuracy to be used for the diagnosis and sero-surveillance analysis of LSD in the target population. © 2007 Elsevier B.V. All rights reserved.
article; Bayes theorem; cattle disease; correlation analysis; diagnostic accuracy; diagnostic procedure; Ethiopia; fluorescent antibody technique; nonhuman; Poxvirus; screening; sensitivity and specificity; serodiagnosis; statistical analysis; virus neutralization; Animals; Antibodies, Viral; Bayes Theorem; Cattle; Female; Fluorescent Antibody Technique, Indirect; Likelihood Functions; Lumpy Skin Disease; Lumpy skin disease virus; Male; Mass Screening; Neutralization Tests; Prevalence; Reproducibility of Results; Sensitivity and Specificity; Bos