Yohannesw M., Gill J.P.S., Ghatak S., Singh D.K., Tolosa T.
School of Veterinary Medicine, Jimma University, College of Agriculture and Veterinary Medicine, PO. Box 307, Jimma, Ethiopia; Department of Veterinary Public Health, Guru Angad Dev Veterinary and Animal Sciences University, PIN 141004, Ludhiana, India; Division of Public Health, Indian Veterinary Research Institute, 243 122, Izatanagar, India
Yohannesw, M., School of Veterinary Medicine, Jimma University, College of Agriculture and Veterinary Medicine, PO. Box 307, Jimma, Ethiopia; Gill, J.P.S., Department of Veterinary Public Health, Guru Angad Dev Veterinary and Animal Sciences University, PIN 141004, Ludhiana, India; Ghatak, S., Department of Veterinary Public Health, Guru Angad Dev Veterinary and Animal Sciences University, PIN 141004, Ludhiana, India; Singh, D.K., Division of Public Health, Indian Veterinary Research Institute, 243 122, Izatanagar, India; Tolosa, T., School of Veterinary Medicine, Jimma University, College of Agriculture and Veterinary Medicine, PO. Box 307, Jimma, Ethiopia
In this study, 241 serum samples from individuals exposed to brucellosis were subjected to the Rose Bengal plate test (RBPT); the titre was estimated by standard tube agglutination test (STAT), with positive > 80 IU/ml. Randomly selected sera (n = 81) were analysed by complement fixation test (CFT): titre > 1:4 was considered positive. Of 241 sera subjected to RBPT and STAT, 177 were negative in both tests; 5 samples tested negative by RBPT but positive by STAT. None was positive by RBPT and negative by STAT. Of 81 sera subjected to CFT, 23 (28.4%) were positive. Both RBPT and CFT found 18 samples positive; 5 samples were positive by CFT and negative by RBPT. Comparison of STAT with CFT showed 13 samples positive by STAT but negative by CFT, and 4 positive by CFT but negative by STAT. The sensitivity and specificity of STAT were 82.6% and 77.6%, respectively, with CFT as gold standard. No test is perfect, and the clinical history coupled with a combination of two or more tests will reduce diagnostic errors.
bacterium antibody; diagnostic agent; fluorescent dye; rose bengal; agglutination test; article; blood; Brucella; brucellosis; comparative study; complement fixation test; cross-sectional study; evaluation study; human; immunology; sensitivity and specificity; standard; Agglutination Tests; Antibodies, Bacterial; Brucella; Brucellosis; Complement Fixation Tests; Cross-Sectional Studies; Fluorescent Dyes; Humans; Rose Bengal; Sensitivity and Specificity