Gari G., Abie G., Gizaw D., Wubete A., Kidane M., Asgedom H., Bayissa B., Ayelet G., Oura C.A.L., Roger F., Tuppurainen E.S.M.
National Animal Health Diagnostic and Investigation Center (NAHDIC), Sebeta, Ethiopia; National Veterinary Institute (NVI), Debre Zeit, Ethiopia; International Agricultural Research Center for Development (CIRAD), Montpellier, France; The Pirbright Institute, Ash Road, Pirbright, Surrey, United Kingdom; School of Veterinary Medicine, Faculty of Medical Sciences, University of the West Indies, Champ Fleurs, Saint Augustine, Trinidad and Tobago
Gari, G., National Animal Health Diagnostic and Investigation Center (NAHDIC), Sebeta, Ethiopia; Abie, G., National Animal Health Diagnostic and Investigation Center (NAHDIC), Sebeta, Ethiopia; Gizaw, D., National Animal Health Diagnostic and Investigation Center (NAHDIC), Sebeta, Ethiopia; Wubete, A., National Animal Health Diagnostic and Investigation Center (NAHDIC), Sebeta, Ethiopia; Kidane, M., National Animal Health Diagnostic and Investigation Center (NAHDIC), Sebeta, Ethiopia; Asgedom, H., National Animal Health Diagnostic and Investigation Center (NAHDIC), Sebeta, Ethiopia; Bayissa, B., National Veterinary Institute (NVI), Debre Zeit, Ethiopia; Ayelet, G., National Veterinary Institute (NVI), Debre Zeit, Ethiopia; Oura, C.A.L., School of Veterinary Medicine, Faculty of Medical Sciences, University of the West Indies, Champ Fleurs, Saint Augustine, Trinidad and Tobago; Roger, F., International Agricultural Research Center for Development (CIRAD), Montpellier, France; Tuppurainen, E.S.M., The Pirbright Institute, Ash Road, Pirbright, Surrey, United Kingdom
The safety, immunogenicity and efficacy of three commercially available vaccines against lumpy skin disease (LSD) in cattle have been evaluated using a combination of vaccine challenge experiments and the monitoring of immune responses in vaccinated animals in the field. The three vaccines evaluated in the study included two locally produced (Ethiopian) vaccines (lumpy skin disease virus (LSDV) Neethling and Kenyan sheep and goat pox (KSGP) O-180 strain vaccines) and a Gorgan goat pox (GTP) vaccine manufactured by Jordan Bio-Industries Centre (JOVAC). The latter vaccine was evaluated for the first time in cattle against LSDV. The Ethiopian Neethling and KSGPO-180 vaccines failed to provide protection in cattle against LSDV, whereas the Gorgan GTP vaccine protected all the vaccinated calves from clinical signs of LSD. There was no significant difference in protective efficacy detected between two dosage levels (P= 0.2, P= 0.25, and P= 0.1 for KSGP, Neethling and Gorgan vaccines, respectively). Additionally, the Gorgan GTP vaccinated cattle showed stronger levels of cellular immune responses measured using Delayed-Type Hypersensitivity (DTH) reactions at the vaccination site indicating higher levels of immunogenicity produced by the GTPV vaccine in cattle, as opposed to the other two vaccines. This study indicated, for the first time, that the Gorgan GTP vaccine can effectively protect cattle against LSDV and that the Neethling and KSGP O-180 vaccine were not protective. The results emphasise the need for molecular characterization of the Neethling and KSGP O-180 vaccine seed viruses used for vaccine production in Ethiopia. In addition, the potency and efficacy testing process of the Ethiopian LSD Neethling and KSGP O-180 vaccines should be re-evaluated. © 2015 Elsevier Ltd.
gorgan goat pox vaccine; live vaccine; lumpy skin disease virus vaccine; neethling and kenyan sheep and goat pox vaccine; unclassified drug; virus vaccine; live vaccine; virus antibody; virus vaccine; animal cell; animal experiment; Article; bovine; cellular immunity; controlled study; cytopathogenic effect; delayed hypersensitivity; depression; drug efficacy; drug safety; drug screening; Ethiopia; eye discharge; fever; fluorescent antibody technique; goatpox; human; immunoassay; immunogenicity; injection site swelling; loss of appetite; lumpy skin disease; lymphadenopathy; malaise; newborn; nonhuman; nose disease; outcome assessment; priority journal; real time polymerase chain reaction; skin edema; skin nodule; skinfold thickness; vaccination; vaccine failure; vaccine production; virus immunity; animal; blood; Capripoxvirus; immunology; lumpy skin disease; Lumpy skin disease virus; sheep; vaccination; veterinary; Animals; Antibodies, Viral; Capripoxvirus; Cattle; Ethiopia; Lumpy Skin Disease; Lumpy skin disease virus; Sheep; Vaccination; Vaccines, Attenuated; Viral Vaccines