Nanotechnology Innovation Centre, Advanced Materials Division, Mintek, Private Bag X3015, Randburg 2125, South Africa; Nanotechnology Innovation Centre, Sensors, Chemistry Department, Rhodes University, P.O. Box 94, Grahamstown 6140, South Africa
Mashazi, P., Nanotechnology Innovation Centre, Advanced Materials Division, Mintek, Private Bag X3015, Randburg 2125, South Africa, Nanotechnology Innovation Centre, Sensors, Chemistry Department, Rhodes University, P.O. Box 94, Grahamstown 6140, South Africa; Vilakazi, S., Nanotechnology Innovation Centre, Advanced Materials Division, Mintek, Private Bag X3015, Randburg 2125, South Africa; Nyokong, T., Nanotechnology Innovation Centre, Sensors, Chemistry Department, Rhodes University, P.O. Box 94, Grahamstown 6140, South Africa
The design of electrochemical immunosensors for the detection of measles-specific antibodies is reported. The measles-antigen modified surface was used as an antibody capture surface. The detection of measles-specific IgG antibodies was accomplished using the voltammetric method and horse-radish peroxidase (HRP) labeled secondary antibody (anti-IgG) as a detecting antibody. The potential applications of the designed immunosensor were evaluated in buffer and serum solutions. The immunosensor exhibited good linearity at concentrations less than 100 ng mL-1 with R2=0.997 and the limit of detection of 6.60 ng mL-1 at 3σ. The potential application of the immunosensor was evaluated in the deliberately infected human and newborn calf serum samples with measles-IgG antibody mimicking real-life samples. The designed electrochemical immunosensor could differentiate between infected and un-infected serum samples as higher catalytic currents were obtained for infected serum samples. © 2013 Elsevier B.V.
Design and evaluations; Electrochemical immunosensors; Horse-radish peroxidase; Labeled-detection; Limit of detection; Measles-antigen; Secondary antibodies; Voltammetric methods; Antigens; Body fluids; Chemical detection; Immunosensors; Antibodies; anti IgG; anti-IgG; antiidiotypic antibody; horseradish peroxidase; immunoglobulin G; virus antibody; virus antigen; animal; article; blood; cattle; chemistry; equipment design; genetic procedures; Horse-radish peroxidase; human; immunoassay; immunology; immunosensor; Labeled-detection; limit of detection; measles; Measles-antigen; Measles-specific antibodies; newborn; virology; Horse-radish peroxidase; Immunosensors; Labeled-detection; Measles-antigen; Measles-specific antibodies; Animals; Antibodies, Anti-Idiotypic; Antibodies, Viral; Antigens, Viral; Biosensing Techniques; Cattle; Equipment Design; Horseradish Peroxidase; Humans; Immunoassay; Immunoglobulin G; Infant, Newborn; Limit of Detection; Measles