Grobusch M.P., Niedrig M., Göbels K., Klipstein-Grobusch K., Teichmann D.
Medical Clinic, Department of Infectious Diseases, Humboldt University, Humboldt, Germany; Robert Koch Institute, Berlin, Germany; German Institute of Human Nutrition, DIfE, Nuthetal, Germany; Division of Clinical Microbiology and Infectious Diseases, National Health Laboratory Service, University of the Witwatersrand, 7 York Road, Parktown 2196 Johannesburg, South Africa
Grobusch, M.P., Medical Clinic, Department of Infectious Diseases, Humboldt University, Humboldt, Germany, Division of Clinical Microbiology and Infectious Diseases, National Health Laboratory Service, University of the Witwatersrand, 7 York Road, Parktown 2196 Johannesburg, South Africa; Niedrig, M., Robert Koch Institute, Berlin, Germany; Göbels, K., Medical Clinic, Department of Infectious Diseases, Humboldt University, Humboldt, Germany; Klipstein-Grobusch, K., German Institute of Human Nutrition, DIfE, Nuthetal, Germany; Teichmann, D., Medical Clinic, Department of Infectious Diseases, Humboldt University, Humboldt, Germany
RT-PCR was used to diagnose dengue virus infections confirmed serologically in 26 returning travellers. RT-PCR was positive for three (75%) of four samples taken on or before day 3 of the illness, for 15 (78.9%) of 19 samples taken between days 4 and 7, and for none of three samples tested on or after day 8 (p 0.0337). When applied early, RT-PCR seems to be a useful tool for the diagnosis of dengue fever. © 2006 Copyright by the European Society of Clinical Microbiology and Infectious Diseases.
immunoglobulin M; monoclonal antibody; virus antigen; adult; antigen detection; article; clinical article; controlled study; dengue; disease course; early diagnosis; female; human; immunofluorescence test; laboratory diagnosis; male; priority journal; quantitative analysis; reverse transcription polymerase chain reaction; serodiagnosis; strain difference; travel; virus detection; virus isolation; Adolescent; Adult; Dengue; Dengue Virus; Early Diagnosis; Female; Humans; Male; Reverse Transcriptase Polymerase Chain Reaction; RNA, Viral; Dengue virus