The primary subcellular localization of Zinc phthalocyanine and its cellular impact on viability, proliferation and structure of breast cancer cells (MCF-7)
Journal of Photochemistry and Photobiology B: Biology
Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, South Africa
The development of curative techniques which are selective for neoplasms is one of the main focal areas in cancer research. The mechanism of cell damage due to Zinc phthalocyanine (ZnPcSmix)-mediated photodynamic therapy (PDT) in a breast cancer cell line (MCF-7) was assessed by inverted light microscopy for morphology, the Trypan blue exclusion assay and adenosine triphosphate (ATP) luminescence assay for cell viability, alamarBlue for proliferation, Lactate Dehydrogenase (LHD) membrane integrity for cytotoxicity and fluorescent microscopy for ZnPcSmix localization. Fluorescent microscopy revealed that ZnPcSmix was localized in both mitochondria and lysosomes, and PDT treated cells showed damaging structural changes and decreased cell viability and proliferation. The light-dependent ZnPcS mix displayed appreciable photosensitivity and the intensity of damage was directly related to its concentration. © 2013 Elsevier B.V. All rights reserved.
adenosine triphosphate; lactate dehydrogenase; phthalocyanine zinc; article; breast cancer; cancer cell; cell damage; cell proliferation; cell structure; cell viability; cellular distribution; controlled study; cytotoxicity; fluorescence microscopy; human; human cell; lysosome; microscopy; mitochondrion; morphology; photodynamic therapy; photosensitivity; photosensitization; priority journal; Biological Transport; Breast Neoplasms; Cell Death; Cell Proliferation; Cell Survival; Humans; Indoles; Intracellular Space; Laser Therapy; MCF-7 Cells; Organometallic Compounds; Photosensitizing Agents; Sulfonic Acids