Synthesis and evaluation of antimicrobial properties of AZO dyes
International Journal of Pharmacy and Pharmaceutical Sciences
Centre for Drug Design and Development, Department of Pharmaceutical Chemistry, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
Objective: To synthesizeazo dyesand evaluate their antimicrobial potential. Methods: A number of azo compounds were synthesized via diazotization of primary aromatic amine and subsequent coupling with naphthols or other coupling partners. The antimicrobial properties of these azo compounds were determined against six microbial species; Staphylococcus aureus(ATCC25923), Escherichia coli (ATCC10231), Mycobacterium smegmatis (clinical strain), Micrococcus luteus(ATCC10240), Pseudomonas aureginosa (ATCC 9027) and the fungusCandida albicans(ATCC10231) using the Kirby-Bauer Standard disc diffusion method. The minimum inhibition concentrations (MIC)were also determined for those compounds that exhibited antimicrobial activity. Results: Two of the azo compounds showed inhibition against microbial agents, with p-NAαN in particular exhibiting very good antimicrobial properties. However, Pseudomonas aureginosa (ATCC 9027) was resistant against all the azo compounds. Conclusion: p-NAαN showed broad spectrum of activity againstStaphylococcus aureus, Escherichia coli, Mycobacterium smegmatis, Micrococcus luteusand the fungal species Candida albicans, with p-ABAαN exhibiting activity against Candida albicans. © 2015, International Journal of Pharmacy and Pharmaceutical Science. All rights reserved.
azo dye; antimicrobial activity; Article; Candida albicans; controlled study; cross coupling reaction; diazotization; disk diffusion; drug synthesis; Escherichia coli; Micrococcus luteus; minimum inhibitory concentration; Mycobacterium smegmatis; nonhuman; Pseudomonas aeruginosa; Staphylococcus aureus; thin layer chromatography