Wang R.-L., Hou Z.P., Wang B., Liu Z.-Q., Fatufe A.A.
Jiangsu Institute of Education, Nanjing 210013, China; Jiangsu Food Science College, Huaian 223003, China; Graduate School, Chinese Academy of Sciences, Beijing 100039, China; Department of Animal Sciences, Obafemi Awolowo University, Ile-Ife, Nigeria
Wang, R.-L., Jiangsu Institute of Education, Nanjing 210013, China, Jiangsu Food Science College, Huaian 223003, China; Hou, Z.P., Graduate School, Chinese Academy of Sciences, Beijing 100039, China; Wang, B., Jiangsu Food Science College, Huaian 223003, China; Liu, Z.-Q., Graduate School, Chinese Academy of Sciences, Beijing 100039, China; Fatufe, A.A., Department of Animal Sciences, Obafemi Awolowo University, Ile-Ife, Nigeria
One hundred and twenty newly-weaned piglets (16-19 days old; 4 pigs per pen; 6 pens per treatment) were used to investigate the effect of feeding galactomannan oligosaccharides (GMOS; from sesbania gum, containing 20% galactose and 15% mannose) on growth performance, serum antibody levels and intestinal microbiota. Five dietary treatments were: basal diet (control), medicated diet (control +110 ppm lincomycin), and three dietary inclusion levels of GMOS (control + 0.1%, 0.2% or 0.3%). The inclusion of either lincomycin or GMOS in the diet did not influence piglet performance (P>0.10), except for a better F/G (P = 0.07, linearly) for the 14-28 day piglets fed the 0.2% GMOS compared with all other treatments. No difference (P>0.05) was observed for faecal consistency score among the dietary treatments during the experiment. On day 4 post-weaning, blood serum levels of IgA (70 vs. 87 mg·dl -1 on control vs. 0.3% GMOS), IgG (285 vs. 401 mgdl' on control vs. 0.3% GMOS) and IgM (155 vs. 273 mg·dl-1 on control vs. 0.3% GMOS) increased linearly (P<0.05) with dietary GMOS level. On day 14 post-weaning, serum IgA and IgM levels were not influenced by feeding GMOS (P>0.05). Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis indicated that medicated diet with 110 ppm lincomycin significantly reduced the diversity of ileal bacteria. Inclusion of GMOS at 0.3% appeared to alter the compositions of both ileal and colonic microbiota. These results suggest that dietary GMOS is a good alternative for dietary antibiotics for the first week post-weaning pigs.
calcium; calcium phosphate dibasic; galactomannan; galactose; immunoglobulin A; immunoglobulin G; immunoglobulin M; lincomycin; mannose; methionine; oligosaccharide; phosphorus; threonine; animal experiment; animal food; antibody blood level; article; body growth; controlled study; corn; denaturing gradient gel electrophoresis; diet; feces analysis; feeding; ileum; immunoglobulin blood level; intestine flora; nonhuman; polymerase chain reaction; scoring system; Sesbania; soybean; swine; weaning; wheat; whey; Sesbania; Suidae