Performance evaluation of two serological tests for contagious bovine pleuropneumonia (CBPP) detection in an enzootic area using a Bayesian framework
Tropical Animal Health and Production
Central Veterinary Laboratory, Km 8, Route de Koulikoro, BP: 2295 Bamako, Mali; CIRAD, Control of Exotic and Emerging Animal Diseases Unit, TA A-15/G, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France; African Union, Interafrican Bureau For Animal Resources, Kenindia Business Park, Museum Hill, Westlands Road, Nairobi, Kenya; CIRAD, Animal and Integrated Risk Management (AGIRs) Unit, (ES), TA A-22/E, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France; CIRAD, SELMET Unit, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France
A Bayesian approach, allowing for conditional dependence between two tests was used to estimate without gold standard the sensitivities of complement fixation test (CFT) and competitive enzyme-linked immunosorbent assay test (cELISA) and the serological prevalence of CBPP in a cattle population of the Central Delta of the Niger River in Mali, where CBPP is enzootic and the true prevalence and animals serological state were unknown. A significant difference (P = 0.99) was observed between the sensitivities of the two tests, estimated at 73.7% (95% probability interval [PI], 63.4-82.7) for cELISA and 42.3% (95% PI, 33.3-53.7) for CFT. Individual-level serological prevalence in the study population was estimated at 14.1% (95% PI, 10.8-16.9). Our results indicate that in enzootic areas, cELISA performs better in terms of sensitivity than CFT. However, negative conditional sensitivity dependence between the two tests was detected, implying that to achieve maximum sensitivity, the two tests should be applied in parallel. © 2011 Springer Science+Business Media B.V.
animal; animal disease; article; Bayes theorem; cattle; cattle disease; comparative study; complement fixation test; contagious pleuropneumonia; enzyme linked immunosorbent assay; epidemiology; evaluation; Mali; methodology; microbiology; sensitivity and specificity; Animals; Bayes Theorem; Cattle; Cattle Diseases; Complement Fixation Tests; Enzyme-Linked Immunosorbent Assay; Mali; Pleuropneumonia, Contagious; Sensitivity and Specificity; Seroepidemiologic Studies; Animalia; Bos; Bovinae; Mycoplasma