Evaluation of the inhibition of carbohydrate hydrolyzing enzymes, the antioxidant activity, and the polyphenolic content of citrus limetta peel extract
Centro de Investigacion y Asistencia en Tecnologia y Diseno Del Estado de Jalisco, A.C., Avenida Normalistas 800, Guadalajara, Mexico; Department of Chemistry, Lagos State University, PMB 0001, Lasu Post Office, Ojo, Lagos, Nigeria; Lancaster Medical Scho
Type 2 diabetes mellitus is one of the most frequent causes of death in Mexico, characterized by chronic hyperglycemia. One alternative strategy for this metabolic abnormality is inhibiting the enzymes responsible for the metabolism of carbohydrates. We evaluated whether the aqueous Citrus limetta peel extract could inhibit the metabolism of carbohydrates. We found that this extract inhibited primarily the enzyme α-amylase by 49.6% at a concentration of 20 mg/mL and to a lesser extent the enzyme α-glucosidase with an inhibition of 28.2% at the same concentration. This inhibition is likely due to the high polyphenol content in the Citrus limetta peel (19.1 mg GAE/g). Antioxidant activity of the Citrus limetta peel demonstrated dose-dependent antioxidant activity, varying from 6.5% at 1.125 mg/mL to 42.5% at 20 mg/mL. The study of these polyphenolic compounds having both antihyperglycemic and antioxidant activities may provide a new approach to the management of type 2 diabetes mellitus. © 2014 Eduardo Padilla-Camberos et al.
alpha glucosidase; amylase; antioxidant; carbohydrate; citrus fruit extract; citrus limetta peel extract; hydrolase; polyphenol derivative; unclassified drug; amylase; antioxidant; plant extract; polyphenol; antioxidant activity; Article; carbohydrate metabolism; concentration response; enzyme activity; enzyme inhibition; hyperglycemia; non insulin dependent diabetes mellitus; antagonists and inhibitors; chemistry; Citrus; Diabetes Mellitus, Type 2; drug effects; human; hyperglycemia; metabolism; Mexico; pathology; Citrus limetta; alpha-Amylases; Antioxidants; Carbohydrate Metabolism; Citrus; Diabetes Mellitus, Type 2; Humans; Hyperglycemia; Mexico; Plant Extracts; Polyphenols