The progressive effects of a high-fat diet on erythrocyte osmotic fragility, growth performance and serum triglyceride and cholesterol levels in Guinea fowl (Numida meleagris) and Muscovy duck (Cairina moschata)
Journal of Animal Physiology and Animal Nutrition
Faculty of Health Sciences, School of Physiology, University of the Witwatersrand, Johannesburg, South Africa
To investigate the progressive effects of a high-fat diet on erythrocyte osmotic fragility, growth performance and serum lipid concentrations in Guinea fowl and Muscovy ducks, 36 Guinea fowl and 36 Muscovy ducks were divided into two groups, for each species, and fed either a standard (STD = commercial poultry feed) or high-fat diet (HFD = commercial poultry feed with 20% palm oil and 2% lard) for up to 12 weeks. After 4, 8 and 12 weeks on the diets, six birds from each group were euthanized and blood samples collected. Osmotic fragility was assessed by measuring the haemoglobin released by erythrocytes placed in serially diluted solutions of phosphate-buffered saline, spectrophotometrically. Serum triglyceride and cholesterol concentrations were also determined. Fragiligrams from erythrocytes from both species of birds on the HFD were not different to those on the STD. However, Muscovy duck erythrocytes were more resistant to haemolysis compared with Guinea fowl erythrocytes. Final body mass and serum triglyceride levels were not significantly different (p > 0.05, ANOVA) between the birds in the HFD and STD groups, for both species of birds. In contrast, serum cholesterol levels were significantly higher in birds on the HFD compared with those on the STD, after 4, 8 and 12 weeks of feeding, for both species of birds. Feeding Guinea fowl and Muscovy ducks a high-fat diet for up to 12 weeks resulted in hypercholesterolaemia but had no effect on final body mass, erythrocyte osmotic fragility or serum triglyceride concentrations in either bird species. © 2014 Blackwell Verlag GmbH.
Aves; Cairina moschata; Numida meleagris; cholesterol; fat intake; triacylglycerol; administration and dosage; analysis; animal; animal food; blood; controlled study; diet; drug effects; duck; erythrocyte; fat intake; Galliformes; growth, development and aging; metabolism; osmotic fragility; randomized controlled trial; species difference; veterinary; Animal Feed; Animal Nutritional Physiological Phenomena; Animals; Cholesterol; Diet; Dietary Fats; Ducks; Erythrocytes; Galliformes; Osmotic Fragility; Species Specificity; Triglycerides