Robson-Ansley P.J., Saini A., Toms C., Ansley L., Walshe I.H., Nimmo M.A., Curtin J.A.
Faculty of Health and Life Sciences, Northumbria University, Newcastle-upon-Tyne, United Kingdom; Department of Physiological Sciences, Stellenbosch University, South Africa; Department of Physiology and Pharmacology, Karolinska Institutet, Sweden; School of Sport, University of Stirling, Stirling, United Kingdom; School of Sport, Exercise and Health, Loughborough, United Kingdom; University of Manchester, Manchester Academic Health Science Centre, University Hospital of South Manchester NHS Foundation Trust, Manchester, United Kingdom
Robson-Ansley, P.J., Faculty of Health and Life Sciences, Northumbria University, Newcastle-upon-Tyne, United Kingdom, Department of Physiological Sciences, Stellenbosch University, South Africa; Saini, A., Department of Physiology and Pharmacology, Karolinska Institutet, Sweden; Toms, C., Faculty of Health and Life Sciences, Northumbria University, Newcastle-upon-Tyne, United Kingdom; Ansley, L., Faculty of Health and Life Sciences, Northumbria University, Newcastle-upon-Tyne, United Kingdom; Walshe, I.H., School of Sport, University of Stirling, Stirling, United Kingdom; Nimmo, M.A., School of Sport, Exercise and Health, Loughborough, United Kingdom; Curtin, J.A., University of Manchester, Manchester Academic Health Science Centre, University Hospital of South Manchester NHS Foundation Trust, Manchester, United Kingdom
The aim of the present study was to examine the relationship between interleukin (IL)-6 concentrations and DNA methylation in the peripheral blood mononuclear cells (PBMCs) of trained runners after a bout of prolonged, strenuous exercise. Eight healthy trained males completed a treadmill run at 60% vVO<inf>2max</inf> for 120 min followed by a 5-km time trial in a fasted condition. Whole blood samples were taken prior to, immediately before and 24 h following exercise. From these samples, PBMCs were isolated for analysis and plasma IL-6 concentrations were measured. The methylation status of DNA extracted from PBMCs was analysed using the Illumina 27k methylation beadchip platform. Global DNA methylation status was unaltered immediately and up to 24 hours following a bout of prolonged exercise in comparison to pre-exercise. Despite no change in global DNA methylation, plasma IL-6 concentrations were significantly related to the DNA methylation status of 11 genes. Our study demonstrates that the methylome is stable, while discovering a novel link between exercise-induced increases in circulating IL-6 and the DNA methylation status of 11 individual genes. Based on our preliminary findings, the mechanisms by which changes in plasma IL-6 concentrations and DNA methylation in response to exercise interact require further study. Copyright © by BIOLIFE, s.a.s.
DNA; interleukin 6; IL6 protein, human; interleukin 6; adult; Article; blood sampling; controlled study; DNA extraction; DNA methylation; endurance training; genetic association; human; human cell; male; normal human; peripheral blood mononuclear cell; protein blood level; protein DNA interaction; running; transcription regulation; treadmill; treadmill exercise; blood; clinical trial; DNA methylation; exercise; metabolism; mononuclear cell; physiology; Adult; DNA Methylation; Exercise; Humans; Interleukin-6; Leukocytes, Mononuclear; Male; Running