UV-visible and electrochemical monitoring of carbon monoxide release by donor complexes to myoglobin solutions and to electrodes modified with films containing hemin
Ecole Nationale Supérieure de Chimie de Paris, Unité de Pharmacologie Chimique et Génétique, UMR CNRS 8151/U INSERM 640, 11 Rue Pierre et Marie Curie, 75231, Paris Cedex 05, France; Department of Chemistry, Rhodes University, Grahamstown 6140, South Africa; Facultad de Química Y Biología, Universidad de Santiago de Chile, Casilla 40, Correo 33, Santiago, Chile; Ecole Nationale Supérieure de Chimie de Paris, Laboratoire de Chimie et Biochimie des Complexes Moléculaires, UMR CNRS 7576, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France
This study reports on the evaluation of the CO donating behavior of tricarbonyl dichloro ruthenium(II) dimer ([Ru(CO)3Cl 2]2) and 1,3-dimethoxyphenyl tricarbonyl chromium (C 6H3(MeO)2Cr(CO)3) complex by UV-visible technique and electrochemical technique. The CO release was monitored by following the modifications of the UV-visible features of MbFe(II) in phosphate buffer solution and the redox features of reduced Hemin, HmFe(II), confined at the surface of a vitreous carbon electrode. In the latter case, the interaction between the hemin-modified electrode and the released CO was seen through the observation of an increase of the reduction current related to the FeIII/FeII redox process of the immobilized porphyrin. While the ruthenium-based complex, ([Ru(CO)3Cl2] 2), depended on the presence of Fe(II) species to release CO, it was found that the chromium-based complex released spontaneously CO. This was facilitated by illuminating and/or simple stirring of the solution containing the complex. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.