Goosen W.J., Cooper D., Warren R.M., Miller M.A., van Helden P.D., Parsons S.D.C.
DST/NRF Centre of Excellence for Biomedical TB Research/MRC Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 19063, Tygerberg, South Africa; Ezemvelo KZN Wildlife, PO Box 25, Mtubatuba, South Africa
Goosen, W.J., DST/NRF Centre of Excellence for Biomedical TB Research/MRC Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 19063, Tygerberg, South Africa; Cooper, D., Ezemvelo KZN Wildlife, PO Box 25, Mtubatuba, South Africa; Warren, R.M., DST/NRF Centre of Excellence for Biomedical TB Research/MRC Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 19063, Tygerberg, South Africa; Miller, M.A., DST/NRF Centre of Excellence for Biomedical TB Research/MRC Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 19063, Tygerberg, South Africa; van Helden, P.D., DST/NRF Centre of Excellence for Biomedical TB Research/MRC Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 19063, Tygerberg, South Africa; Parsons, S.D.C., DST/NRF Centre of Excellence for Biomedical TB Research/MRC Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 19063, Tygerberg, South Africa
We evaluated commercially available bovine enzyme linked immunosorbent assays (ELISA) and a human IP-10 ELISA to measure IP-10, MIG, MCP-1, MCP-2, MCP-3 and IL1-RA in buffalo plasma in order to identify sensitive markers of the immune response to Mycobacterium bovis-specific peptides. Additionally, we found that all coding mRNA sequences of these cytokines showed very high homology with their homologues in domestic cattle (97-99%) as did the derived amino acid sequences (97-99%). This high sequence homology between cattle and buffaloes supports the use of bovine ELISAs for the detection these cytokines in buffaloes. MCP-1 concentration showed a positive correlation with that of IFN-γ (p=. 0.0077) and appears to occur in far greater abundance in buffaloes when compared to humans. Using a bovine IP-10 ELISA, levels of this cytokine were found to be significantly increased in antigen-stimulated blood samples from M. bovis test positive buffaloes (p < 0.0001) and IP-10 was detected in far greater abundance than IFN-γ. Measurement of IP-10 with this ELISA may prove to be a sensitive marker of M. bovis infection in African buffaloes. © 2014 Elsevier B.V.
gamma interferon; gamma interferon inducible protein 10; interleukin 1 receptor accessory protein; messenger RNA; monocyte chemotactic protein 1; monocyte chemotactic protein 2; monocyte chemotactic protein 3; biological marker; CXCL9 chemokine; gamma interferon inducible protein 10; interleukin 1 receptor blocking agent; monocyte chemotactic protein; African buffalo; amino acid sequence; Article; blood sampling; bovine tuberculosis; cellular immunity; domestic cattle; enzyme linked immunosorbent assay; evaluation study; gene expression assay; immune response; nonhuman; sequence homology; animal; blood; bovine; buffalo; cellular immunity; immunology; microbiology; mycobacteriosis; Mycobacterium bovis; nonparametric test; procedures; veterinary; Bos; Bos taurus; Bovinae; Bubalus; Mycobacterium bovis; Syncerus caffer; Animals; Biological Markers; Buffaloes; Cattle; Chemokine CXCL10; Chemokine CXCL9; Enzyme-Linked Immunosorbent Assay; Immunity, Cellular; Interleukin 1 Receptor Antagonist Protein; Monocyte Chemoattractant Proteins; Mycobacterium bovis; Mycobacterium Infections; Statistics, Nonparametric