Performance of a prototype malaria rapid diagnostic test versus thick film microscopy among HIV-positive subjects in rural Rakai, Uganda
Transactions of the Royal Society of Tropical Medicine and Hygiene
Division of Infectious Diseases, Johns Hopkins Medical Institutions, 1830 East Monument Street #401, Baltimore, MD 21287, United States; Rakai Health Sciences Program, Kalisizo, Rakai District, Uganda; Clinical Monitoring Research Program, SAIC-Frederick Inc., NCI-Frederick, Frederick, MD 21702, United States; Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 6610 Rockledge Drive, Bethesda, MD 20892, United States; Johns Hopkins University School of Medicine, 855 N. Wolfe St., Fifth Floor, Baltimore, MD 21205, United States
In this study, we report the performance of a prototype malaria rapid diagnostic test, Malaria F-test (MFT), compared with thick blood films from HIV-positive Ugandans undergoing malaria testing. In total, 21/154 samples (13.6%) were concordantly positive by both thick film and MFT and 129/154 samples (83.8%) were concordantly negative; 1 sample (0.6%) was thick film-positive but MFT-negative and 3 samples (1.9%) were thick film-negative but MFT-positive. The sensitivity of MFT was 95.5% (95% CI 77.2-99.9%) compared with thick film microscopy and the specificity was 97.7% (95% CI 93.5-99.5%). MFT was simple, rapid and effective for detection of Plasmodium falciparum among HIV-positive subjects in a rural, malaria-endemic African setting.
artemether plus benflumetol; adolescent; adult; article; child; controlled study; diagnostic test; drug efficacy; female; human; Human immunodeficiency virus infected patient; incidence; intermethod comparison; lymphocyte count; major clinical study; malaria; malaria F test; male; microscopy; Plasmodium falciparum; sensitivity and specificity; T lymphocyte; Uganda; Adolescent; Adult; Child; Child, Preschool; Diagnostic Tests, Routine; Female; HIV Seropositivity; Humans; Malaria, Falciparum; Male; Middle Aged; Plasmodium falciparum; Sensitivity and Specificity; Uganda; Young Adult; Plasmodium falciparum