Evaluation of wound healing activity of ethanolic extract of Lantana camara in streptozotocin induced diabetic rats
International Journal of Pharmacy and Pharmaceutical Sciences
Dept. of Clinical Pharmacology, International Medical and Technological University, Dar-Es- Salaam, Tanzania; Dept. of Pharmacology, Sapthagiri Institute of Medical Science and Research Center, Bangalore, India; Dept. of Pharmacology, Kasturba Medical College, Manipal University, India; Dept. of Pharmacology, Melaka Manipal Medical College, Manipal University, India; CSIR- SRF Department of Biotechnology, KL University, Guntur, India; Dept. of Biochemistry, International Medical and Technological University, Dar-Es- Salaam, Tanzania
Background: Lantana camara (Verbanacea) is a commonly available medicinal plant throughout India. Wound healing property of the plant in various wound models has been studied. Thorough literature survey revealed that the wound healing property of Lantana camara in diabetic wound was not studied. This study was aimed to evaluate the wound healing property of Lantana camara in diabetic rats. Methods: Group-1 rats served as normal control in which excision wound was created in normal, non-diabetic rats and wound was topically applied with vehicle. To induce diabetes mellitus in group 2-5, a single injecting of streptozotocin (45 mg/kg, i.p.) prepared by dissolving in 0.9% ice cold citrate buffer was given. Excision wound was inflicted in the back of the rats. Group-2 was the diabetic control in which diabetic rats received vehicle ointment topically. Group 3, 4 and 5 were the test drug groups in which diabetic rats were topically applied ethanolic extract of Lantana camara in three doses 10%, 15% and 20% respectively. Wound healing parameters such as percentage of wound contraction rate and epithelialization period were observed. Data was analyzed using SPSS software by one way ANOVA and the statistical significance was fixed as p < 0.005. Results: There was a delay in wound healing in diabetic rats compared to non-diabetic rats. The extract showed dose dependent increase in wound contraction rate and hastened the epithelialization period. Extracts enhanced contraction rate only during later phase of wound healing process. High dose (20%) extract showed maximum healing effect. Conclusion: Topical application of ethanolic extract of Lantana camara showed dose dependent wound healing activity in diabetic rats.
alkaloid; flavonoid; Lantana camara extract; saponin; tannin; triterpene; animal experiment; animal model; article; controlled study; drug megadose; epithelization; male; nonhuman; rat; streptozotocin-induced diabetes mellitus; wound contraction; wound healing