Evaluation of in vitro antioxidant effect of new mono and diselenides
Departmento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, CEP 97105-900, Brazil; Department of Biochemistry, Federal University of Technology, FUTA Road, Off Ilesha Road, PMB 704 Akure, Ondo State, Nig
This study was designed to examine the antioxidant activity in vitro of novel mono- and diselenide compounds. We compared whether the formation of p-methyl-selenol from compounds 1-phenyl-3-(p-tolylselanyl)propan-2-amine (C1) and 1,2-dip-tolyldiselenide (C4) and o-methoxy-selenol from compounds 1-(2-methoxyphenylselanyl)-3-phenylpropan-2-amine (C2) and 1,2-bis(2-methoxyphenyl)diselenide (C3) may be involved in their antioxidant effects. The compounds were tested against Fe(II) and sodium nitroprusside (SNP)-induced lipid peroxidation in rat brain and liver homogenates. Likewise, the antioxidant capacity of the compounds was assessed by their ability to decolorize the DPPH radical as well as the Fe(II) chelating assay through the reduction of molybdenum(VI) (Mo6+) to molybdenum(V) (Mo5+). This colorimetric assay was also used to quantify thiol peroxidase (GPx) and oxidase activity and thioredoxin reductase (TrxR) activity. The results showed that the novel selenide compounds inhibit the thiobarbituric acid reactive species (TBARS) induced by different pro-oxidants, but the monoselenides effects were significant only at concentrations higher than the concentrations of the diselenides. Similarly, the total antioxidant activity was higher in the diselenides. Moreover, GPx and TrxR activity was only observed for the diselenides, which indicates that these compounds are more stable selenol molecules than monoselenides. © 2013 Elsevier Ltd.
1 (2 methoxyphenylselanyl) 3 phenylpropan 2 amine; 1 phenyl 3 (4 tolylselanyl)propan 2 amine; 1,2 bis (2 methoxyphenyl)diselenide; 1,2 bis 4 tolyldiselenide; antioxidant; nitroprusside sodium; oxidoreductase; peroxidase; thiobarbituric acid reactive substance; thiol peroxidase; thioredoxin reductase; unclassified drug; animal cell; animal experiment; animal tissue; antioxidant activity; article; brain homogenate; chelation; colorimetry; controlled study; drug effect; enzyme activity; in vitro study; lipid peroxidation; liver homogenate; male; nonhuman; rat; Animals; Antioxidants; Brain; Glutathione Peroxidase; Lipid Peroxidation; Liver; Male; NADP; Organoselenium Compounds; Oxidation-Reduction; Rats; Rats, Wistar; Thiobarbituric Acid Reactive Substances; Thioredoxin Reductase 1; Rattus