Department of Virology, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria; WHO National Polio Laboratory, University of Ibadan, Ibadan, Oyo State, Nigeria
Adeniji, J.A., Department of Virology, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria, WHO National Polio Laboratory, University of Ibadan, Ibadan, Oyo State, Nigeria; Faleye, T.O.C., Department of Virology, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria
There has been under-reporting of nonpolio enterovirus species Cs (NPESCs) in Nigeria despite the fact that most isolates recovered from the Nigerian vaccine derived poliovirus serotype 2 (VDPV2) outbreak were recombinants with nonstructural region of NPESC origin. It has been suggested that cell lines included in enterovirus isolation protocols might account for this phenomenon and this study examined this suggestion.Fifteen environmental samples concentrated previously and analysed using L20B and RD cell lines as part of the poliovirus environmental surveillance (ES) program in Nigeria were randomly selected and inoculated into two cell lines (MCF-7 and LLC-MK2). Isolates were identified as enteroviruses and species C members using different RT-PCR assays, culture in L20B cell line and sequencing of partial VP1.Forty-eight (48) isolates were recovered from the 15 samples, 47 (97.9%) of which were enteroviruses. Of the enteroviruses, 32 (68.1%) belonged to enterovirus species C (EC) of which 19 (40.4%) were polioviruses and 13 (27.7%) were NPESC members. All 13 NPESC isolates were recovered on MCF-7.Results of the study show that NPESCs are circulating in Nigeria and their under-reporting was due to the combination of cell lines used for enterovirus isolation in previous reports. © 2014 Elsevier B.V.
protein VP1; virus RNA; Article; cell line; controlled study; Enterovirus; gene sequence; human; human cell; L20B cell line; LLC MK2 cell line; MCF 7 cell line; Nigeria; nonhuman; nonpolio enterovirus species C; Poliomyelitis virus; priority journal; reverse transcription polymerase chain reaction; species diversity; species identification; virus identification; virus isolation; cell line; classification; DNA sequence; Enterovirus; genetics; genotype; isolation and purification; microbiology; molecular genetics; procedures; virus culture; Cell Line; Enterovirus; Environmental Microbiology; Genotype; Humans; Molecular Sequence Data; Nigeria; Reverse Transcriptase Polymerase Chain Reaction; RNA, Viral; Sequence Analysis, DNA; Virus Cultivation