Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Michael Okpara University of Agriculture, Umudike, Nigeria; Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, N
Madubuike, K.G., Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Michael Okpara University of Agriculture, Umudike, Nigeria; Yusuf, N.O., Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, Nigeria; Robinson, E.S., Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, Nigeria
The antioxidant activity of Jatropha tanjorensis leaf methanolic extraxt was investigated following different antioxidant models. The antioxidant methods used include: in vitro 2, 2-diphenylpicrylhydrazine (DPPH) spectrophotometric assay and in vivo assay of serum malondialdehyde, superoxide dismutase and catalase activity. The extract (50–400 μg/ml concentration) produced in a concentration-dependent manner, significant (p < 0. 05) antioxidant effect in the DPPH spectrophotometric assay. In the in vivo antioxidant tests, 400 mg/kg of the extract evoked significant (p < 0. 05) increase in serum superoxide dismutase and catalase activity and also significantly decreased the level of malondialdehyde when compared with the control group. These findings suggest that J. tanjorensis possess potent antioxidant property, which may be responsible for some of the reported pharmacological actions of the plant. © 2015, International Journal of Pharmacognosy and Phytochemical Research. All Rights Reserved.
antioxidant; ascorbic acid; catalase; Jatropha tanjorensis extract; malonaldehyde; plant extract; superoxide dismutase; unclassified drug; acute toxicity; animal experiment; antioxidant activity; Article; controlled study; dose response; down regulation; drug dose comparison; drug screening; enzyme activity; female; in vitro study; in vivo study; Jatropha; Jatropha tanjorensis; lipid peroxidation; male; nonhuman; plant leaf; rat; spectrophotometry