Evaluation of hepatotoxicity and clastogenicity of carbofuran in male Wistar rats
Food and Chemical Toxicology
Cancer Research and Molecular Biology Laboratories, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria
Carbofuran based pesticides have gained wide usage in Nigeria recently. Consequently, animals and human populations are exposed to them in the environment. Information on in vivo toxicity of carbofuran in experimental models is scanty. The present study therefore examined the hepatotoxicity and clastogenic effects of carbofuran in rats. Male Wistar rats were exposed to carbofuran (p.o) at 0-5mg/kg bw for 5weeks. Carbofuran induced significant (p<0.05) increase in the serum activity of gamma-glutamyltransferase when compared with the negative control, but not activity of serum alanine and aspartate aminotransferases. It also significantly (p<0.05) induced micronucleated polychromatic erythrocytes formation in the bone marrow as compared with the control. The level of induction is dose dependent in both cases. In addition, there was significant (p<0.05) higher number of hepatic cells in the cell/mm2 assay for the group treated with carbofuran. Histopathological analysis of liver samples from the treated groups revealed lesions ranging from general congestion (portal, central venous and sinusoidal), mild periportal cellular infiltration, diffused sinusoidal congestion and hepatic necrosis to severe congestion. Findings from this study suggest that carbofuran has clastogenic and hepatotoxic effects in rats. It therefore may constitute an environmental health risks in individuals so exposed. © 2013 Elsevier Ltd.
carbofuran; alanine aminotransferase; aspartate aminotransferase; carbofuran; gamma glutamyltransferase; animal cell; animal experiment; animal tissue; article; cell assay; cell count; cell infiltration; chemical parameters; clastogenicity; controlled study; disease severity; enzyme activity; enzyme blood level; genotoxicity; health hazard; histopathology; liver cell; liver necrosis; liver toxicity; male; nonhuman; rat; alanine aminotransferase blood level; animal model; Article; aspartate aminotransferase blood level; gamma glutamyl transferase blood level; liver weight; long term exposure; mutagenesis; weight change; Alanine aminotransferase; Carbamate; Carbofuran; Gamma gluthamyltransferase; Micronucleated polychromatic erythrocytes (mPCEs); Animals; Carbofuran; Liver; Male; Mutagens; Rats; Rats, Wistar; Rattus norvegicus