Spermatotoxic, cytotoxic and genotoxic evaluation of aqueous extract of Ocimum gratissimum in albino mice
Open Access Journal of Medicinal and Aromatic Plants
Department of Pure and Applied Biology, Ladoke Akintola University of Technology, P.M.B. 4000, Ogbomoso, Nigeria; Cell Biology and Genetics Unit, Department of Zoology, University of Ibadan, Ibadan, Nigeria; Environmental Unit, Department of Pure and Appl
Ocimum gratissimum (Lamiaceae) is an alternative medicine as well as food condiment in African and Asian countries. Information on its safe consumption is important considering its wide utilization. The potential effects of aqueous extract of this plant on cell division, chromosome structure and sperm morphology in mice were investigated. The Inductions of chromosomal aberrations and abnormal sperm cells were not dose dependent. However, these were significantly different (p ≤ 0.05) in male mice at 8% and at all concentrations except at 10% for the bone marrow metaphase and sperm morphology tests, respectively. Aberrant sperm cell in form of tail folded over head was observed most. These observations suggest possible mutagenic activity of the aqueous extract of O. gratissimum in albino mice.
cyclophosphamide; Ocimum gratissimum extract; albino; animal cell; animal experiment; animal model; aqueous solution; article; bone marrow cell; chromosome aberration; concentration (parameters); cytotoxicity; female; genotoxicity; male; metaphase chromosome; mouse; mutagenic activity; nonhuman; semen abnormality; spermatozoon abnormality; spermatozoon head; spermatozoon tail