Mine M., Chishala S., Makhaola K., Tafuma T.A., Bolebantswe J., Merrigan M.B.
Botswana Harvard Reference Laboratory, National Health Laboratory, Gaborone, Botswana; Department of HIV/AIDS Prevention, Care Ministry of Health, Gaborone, Botswana; Family Health International 360, Gaborone, Botswana
Mine, M., Botswana Harvard Reference Laboratory, National Health Laboratory, Gaborone, Botswana; Chishala, S., Botswana Harvard Reference Laboratory, National Health Laboratory, Gaborone, Botswana; Makhaola, K., Botswana Harvard Reference Laboratory, National Health Laboratory, Gaborone, Botswana; Tafuma, T.A., Department of HIV/AIDS Prevention, Care Ministry of Health, Gaborone, Botswana; Bolebantswe, J., Department of HIV/AIDS Prevention, Care Ministry of Health, Gaborone, Botswana; Merrigan, M.B., Family Health International 360, Gaborone, Botswana
The study assessed the performance of rapid HIV testing with whole blood using Kehua Bio-engineering HIV (1 + 2) and Uni-Gold HIV test kits by trained and certified lay counselors, offered to female sex workers and men who have sex with men during the 2012 survey fieldwork. The results of rapid HIV testing were compared with enzyme-linked immunosorbent assay testing performed in a parallel algorithm at the HIV Reference Laboratory. The sensitivity and the specificity of rapid HIV testing were high for men who have sex with men and female sex workers, with 98.1% and 100%, and 98.2% and 98.5%, respectively. Misclassifications occurred with rapid testing. Copyright © 2014 Wolters Kluwer Health, Inc. All rights reserved.
Human immunodeficiency virus antibody; adult; Article; bioengineering; blood analysis; Botswana; diagnostic error; diagnostic test accuracy study; disease surveillance; enzyme linked immunosorbent assay; female; health care personnel; health survey; HIV rapid test; human; Human immunodeficiency virus 1; Human immunodeficiency virus 2; Human immunodeficiency virus infection; lay counselor; major clinical study; male; men who have sex with men; priority journal; prostitution; sensitivity and specificity; adolescent; comparative study; cross-sectional study; diagnostic test; evaluation study; HIV Infections; hospital information system; male homosexuality; middle aged; paramedical personnel; procedures; young adult; Adolescent; Adult; Allied Health Personnel; Botswana; Cross-Sectional Studies; Diagnostic Tests, Routine; Female; HIV Infections; Homosexuality, Male; Humans; Male; Middle Aged; Point-of-Care Systems; Sensitivity and Specificity; Sex Workers; Young Adult