Department of Pharmacology and Toxicology, National Institute for Pharmaceutical Research and Development (NIPRD), Abuja, Nigeria; Directorate of Pharmaceutical Services, Ministry of Health, Damaturu, Yobe State, Nigeria; Department of Medicinal Plant Res
Adzu, B., Department of Pharmacology and Toxicology, National Institute for Pharmaceutical Research and Development (NIPRD), Abuja, Nigeria, Laboratório de Farmacologia, Faculdade de Medicina, Universidade Federal de Mato Grosso (UFMT), Cuiabá, Brazil; Amizan, M.B., Directorate of Pharmaceutical Services, Ministry of Health, Damaturu, Yobe State, Nigeria; Okhale, S.E., Department of Medicinal Plant Research and Traditional Medicine, NIPRD, Abuja, Nigeria
Ethnopharmacological relevance Xeromphis nilotica (Stapf) Keay (Rubiaceae), popularly known as 'barbaji' (in Nigeria), is a lowland shrub that grows wild in tropical areas of Africa and Asia. The plants extract is used for the treatment of various diseases in folk medicine including pain related ailments. Important bioactive constituents have been isolated from the plant among them are coumarin, alkaloids, flavonoids, saponins, and terpenes. This study is aimed to evaluate the analgesic and anti-inflammatory efficacy of standardised aqueous extract of the plant using in vivo models of pain and inflammation in mice and rats.Materials and methods Aqueous extract of Xeromphis nilotica root bark was prepared and standardised using HPLC technique. Three dose levels (25, 100 and 400 mg/kg) of the extract were used, administered orally to laboratory mice and rats. Acetylsalicylic acid (100 mg/kg, p.o.) was used as the positive control. Nociception was induced in laboratory rodents: chemically using acetic acid and formalin, and mechanically using analgesy meter; while inflammation was induced using fresh raw egg albumin.Results The extract showed 11 constituents peak profiles in the HPLC analysis. The extract alleviates mice response to acetic acid-induced writhing, analgesy-meter and formalin tests. It significantly decreased the oedema induced by egg albumin induced inflammation, but failed to show significant effect beyond 80 min of the test.Conclusion The extract has antinociceptive effect and short acting anti-inflammatory activities. The results justify its usage in the treatment of pain and inflammatory conditions, and also provided evidence of its potential as source of new pain relief drug prototype. © 2014 Elsevier Ireland Ltd. All rights reserved.
acetic acid; acetylsalicylic acid; analgesic agent; antiinflammatory agent; formaldehyde; ovalbumin; plant extract; unclassified drug; Xeromphis nilotica extract; analgesic agent; antiinflammatory agent; plant extract; analgesic activity; animal experiment; animal model; antiinflammatory activity; Article; controlled study; drug efficacy; drug screening; experimental mouse; experimental rat; female; high performance liquid chromatography; in vivo study; inflammation; male; mouse; nociception; nonhuman; pain; rat; animal; bark; chemistry; Liliaceae; Wistar rat; Analgesics; Animals; Anti-Inflammatory Agents; Chromatography, High Pressure Liquid; Female; Liliaceae; Male; Mice; Plant Bark; Plant Extracts; Rats; Rats, Wistar