Evaluation of antibacterial activity of leaf and stem extracts of Combretum calobotrys
Journal of Chemical and Pharmaceutical Research
Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria; Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, En
Antibacterial activity of the extracts and fractions of leaf and stem of Combretum calobotrys Engl. & Diels (Combretaceae) against clinical isolates of Bacillus subtilis, Pseudomonas aeruginosa, Salmonella paratyphi, Eschericia coli, Staphylococcus aureus and Klebsiella pneumonia were evaluated using the agar diffusion method. Results showed that with the exception of the stem methanol fraction, all the extracts and fractions, elicited antibacterial activity. The leaf methanol fraction had minimum inhibitory concentration (MIC) of 0.32 mg/ml against K. pneumoniae while the stem ethylacetate fraction had MIC of 0.46 and 0.78 mg/ml against P. aeruginosa and S. aureus respectively. The results justify the ethnomedicinal use of C. calobotrys in Southeastern Nigerian to manage bacterial infections.
acetic acid ethyl ester; Combretum calobotrys extract; gentamicin; methanol; plant extract; unclassified drug; agar diffusion; antibacterial activity; antibiotic sensitivity; article; Bacillus subtilis; bacterium isolate; Combretum; Combretum calobotrys; Escherichia coli; Klebsiella pneumoniae; minimum inhibitory concentration; Nigeria; nonhuman; plant leaf; plant stem; Pseudomonas aeruginosa; Salmonella paratyphi; Staphylococcus aureus; traditional medicine