Safety evaluation of Hymenocardia acida leaf extracts in rats and mice
Journal of Chemical and Pharmaceutical Sciences
Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, Nigeria; ARDRI, University of Fort Hare, Alice 5700, South Africa; Department of Botany, University of Fort Hare, Alice 5700, South Africa; Department of Chemistry, University of Lagos
Acute and sub-acute effects of the aqueous extracts of the leaves of Hymenocardia acida Tul. (Hymenocardiaceae) were studied in mice and rats. In the acute toxicity test, H. acida extract (400-3200 mg/kg) did not cause mortality, behavioural changes, or alteration in physiological activities in mice after 48h of administration. Sub-acute test indicated no significant change in the packed cell volume (PCV), hemoglobin concentration (HB), red blood cell count (RBC) and the erythrocytes indices. A significant increase in white blood cell count (WBC) and decrease in the level of platelets at (200-800 mg/kg) was observed. Changes in the levels of total protein, albumin, globulin and the electrolytes not significant but there was decreased total bilirubin, conjugated bilirubin, unconjugated bilirubin, creatinine, urea, p-glucose, inorganic phosphorus, alkaline phosphatase (ALP), aspartate amino transferase (AST), gamma-glutamyl transferase (GGT) and alanine amino transferase [ALT]. No histopathological changes were observed in all the organs, though there was a significant reduction in their weights.
alanine aminotransferase; albumin; alkaline phosphatase; aspartate aminotransferase; bilirubin; creatinine; electrolyte; gamma glutamyltransferase; globulin; hemoglobin; Hymenocardia acida extract; plant extract; unclassified drug; alkaline phosphatase blood level; animal experiment; article; behavior change; controlled study; erythrocyte; erythrocyte count; gamma glutamyl transferase blood level; hematocrit; hemoglobin blood level; histopathology; leukocyte count; male; mortality; nonhuman; plant leaf; rat; thrombocyte count; Hymenocardia acida; Mus; Rattus