Adebajo A.C., Odediran S.A., Nneji C.M., Iwalewa E.O., Rukunga G.M., Aladesanmi A.J., Gathirwa J.W., Ademowo O.G., Olugbade T.A., Schmidt T.J., Verspohl E.J.
Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile Ife 00235, Nigeria; Institute of Pharmaceutical Biology and Phytochemistry, University of Münster, Münster, Germany; Department of Pharmacology, Institute of Pharmaceutical
Adebajo, A.C., Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile Ife 00235, Nigeria, Institute of Pharmaceutical Biology and Phytochemistry, University of Münster, Münster, Germany, Department of Pharmacology, Institute of Pharmaceutical and Medicinal Chemistry, Westfälische Wilhelms-Universität, Munster, Germany; Odediran, S.A., Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile Ife 00235, Nigeria; Nneji, C.M., Institute of Advanced Medical Research and Training, University of Ibadan, Nigeria; Iwalewa, E.O., Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile Ife 00235, Nigeria; Rukunga, G.M., Centre for Traditional Medicine and Drug Research, Kenya Medical Research Institute, Nairobi, Kenya; Aladesanmi, A.J., Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile Ife 00235, Nigeria; Gathirwa, J.W., Centre for Traditional Medicine and Drug Research, Kenya Medical Research Institute, Nairobi, Kenya; Ademowo, O.G., Institute of Advanced Medical Research and Training, University of Ibadan, Nigeria; Olugbade, T.A., Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile Ife 00235, Nigeria; Schmidt, T.J., Institute of Pharmaceutical Biology and Phytochemistry, University of Münster, Münster, Germany; Verspohl, E.J., Department of Pharmacology, Institute of Pharmaceutical and Medicinal Chemistry, Westfälische Wilhelms-Universität, Munster, Germany
Methanolic extract and chromatographic fractions of Gongronema latifolium were tested against clinical isolates of Plasmodium falciparum-, P. yoelii nigeriensis-infected mice, chloroquine-sensitive (D6) and chloroquine-resistant (W2) P. falciparum clones. The isolates, characterized as a 1:1 mixture of α-amyrin and β-amyrin cinnamates (1a/1b), lupenyl cinnamate (2) and lupenyl acetate (3), were assayed using the clones. Extract, most active vacuum liquid and column chromatographic fractions had respective ED50 values of 120.85, 32.03, 25.62 mg.kg-1 and IC50 of 36.27, 9.45, 7.05 μg.mL-1, against W2 clones. Lupenyl acetate had 18.96 μg.mL-1, indicating synergistic action of the constituents. Results justified its ethnomedical use for treating malaria. © 2013 Taylor & Francis Group, LLC.