In vitro evaluation of effects of two ghanaian plants relevant to wound healing
Department of Pharmacognosy, Faculty of Pharmacy, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana; Pharmacognosy Research Laboratories, Department of Pharmacy, King's College London, 150 Stamford Street, London SE1 9NH, United Kingdom; Centre for Pharmacognosy and Phytotherapy, School of Pharmacy, University of London, 29/39 Brunswick Square, London WC1N 1AX, United Kingdom
Commelina diffusa and Spathodea campanulata are used as wound-healing agents in Ashanti traditional medicine in Ghana. The methanol extracts of Commelina diffusa herb and Spathodea campanulata bark showed some level of antimicrobial activity with C. diffusa exhibiting selective antifungal activity against Trichophyton species. The extracts reduced the peroxidation of bovine brain extract with an IC50 value of 1.39 mg/mL and 0.24 mg/mL, respectively. In addition the extracts also exhibited significant antioxidant activity by protecting MRC-5 cells from hydrogen peroxide induced oxidant injury at concentrations between 1 μg/mL and 10 μg/mL. The extracts showed no inhibition of NF-κB at 100 μg/mL. The antioxidant activities and antimicrobial activities suggest that the use of the plants in wound healing may be based on antioxidant and antiseptic effects of its constituents. Copyright © 2006 John Wiley & Sons, Ltd.
brain extract; hydrogen peroxide; immunoglobulin enhancer binding protein; plant extract; antifungal activity; antimicrobial activity; antioxidant activity; article; bark; cell line; Commelina; controlled study; Ghana; IC 50; in vitro study; nonhuman; peroxidation; Trichophyton; wound healing; Animals; Antioxidants; Bacteria; Bignoniaceae; Brain; Cattle; Cells, Cultured; Commelina; Fungi; Hydrogen Peroxide; Inhibitory Concentration 50; Methanol; Microbial Sensitivity Tests; Phytotherapy; Plant Extracts; Wound Healing; Wounds and Injuries; Bos taurus; Commelina diffusa; Fungi; Spathodea campanulata; Trichophyton