Kenechukwu F.C., Umeyor C.E., Momoh M.A., Ogbonna J.D.N., Chime S.A., Nnamani P.O., Attama A.A.
Department of Pharmaceutics, University of Nigeria, Nsukka 410001, Nigeria; Department of Pharmaceutics and Pharmaceutical Technology, Nnamdi Azikiwe University, Awka, Nigeria; Department of Pharmaceutical Technology and Industrial Pharmacy, University of
Kenechukwu, F.C., Department of Pharmaceutics, University of Nigeria, Nsukka 410001, Nigeria; Umeyor, C.E., Department of Pharmaceutics and Pharmaceutical Technology, Nnamdi Azikiwe University, Awka, Nigeria; Momoh, M.A., Department of Pharmaceutics, University of Nigeria, Nsukka 410001, Nigeria; Ogbonna, J.D.N., Department of Pharmaceutics, University of Nigeria, Nsukka 410001, Nigeria; Chime, S.A., Department of Pharmaceutical Technology and Industrial Pharmacy, University of Nigeria, Nsukka 410001, Nigeria; Nnamani, P.O., Department of Pharmaceutics, University of Nigeria, Nsukka 410001, Nigeria; Attama, A.A., Department of Pharmaceutics, University of Nigeria, Nsukka 410001, Nigeria
Purpose: To formulate solidified reverse micellar solutions (SRMS)-based solid lipid microparticles (SLMs) using homolipid from Capra hircus, and evaluate its suitability for the delivery of gentamicin. Methods: SLMs were formulated by melt-emulsification using SRMS (15 % w/w Phospholipon® 90G in 35 % w/w Capra hircus), PEG 4000 and gentamicin (1.0, 2.0 and 3.0 % w/w), and characterized with respect to size, morphology, encapsulation efficiency (EE) and pH-dependent stability. In vitro release of gentamicin from the SLMs was performed in phosphate buffer (pH 7.4) while bioevaluation was carried out using clinical isolates of Pseudomonas aeruginosa and Staphylococcus aureus. Results: Stable and discrete SLMs of size range 1.47 ± 0.02 to 3.55 ± 0.09 μm were obtained. The SLMs showed a biphasic pattern of drug release and exhibited time-dependent and capacity-limited bioactivity. Overall, SLMs containing 2 % w/w SRMS, 3 % w/w gentamicin and PEG 4000 entrapped the highest amount of drug, released 99 % of drug and gave the highest inhibitory zone diameter (IZD) against the organisms within 420 min, while plain gentamicin gave the least. Conclusion: SRMS-based SLMs prepared with homolipid from Capra hircus offers a suitable delivery system for gentamicin. © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. All rights reserved.