Preparative isolation of bio-markers from the leaf exudate of Aloe ferox ("aloe bitters") by high performance counter-current chromatography
Department of Pharmaceutical Sciences, Tshwane University of Technology, Pretoria, South Africa
One of the most crucial factors determining the safety and efficacy of any herbal medicine or natural product-based formulation is the quality of the raw material. The absence of readily available bio-markers (standards) is one of the hurdles which need to be overcome to develop robust and effective quality control protocols. Aloe ferox Mill. is a most coveted ethnomedicinally import plant indigenous to South Africa. A. ferox has been used since ancient times in folk medicine and recently it has gained popularity as an ingredient in cosmetic formulations and food supplements. This study aimed to develop a superior method for the isolation of bio-markers from "aloe bitters" (exudate) obtained from A. ferox. For separation by HPCCC the solvent system comprising of EtOAc/n-BuOH/H2O (3.5:1.5:5, v/v/v) was used in reversed phase mode. By this method, and only in one run, eight bio-markers were separated and isolated on semi-preparative scale including aloesin, aloeresin C, aloeresin A, 5-hydroxyaloin, aloin B, aloinoside B, aloin A and aloinoside A. The isolation of bio-active molecules from A. ferox (Cape aloes) is presented to illustrate the efficiency and advantages of high performance counter-current chromatography (HPCCC). © 2014 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved.
5 hydroxyaloin; aloeresin a; aloeresin c; aloesin; aloin; aloinoside a; aloinoside b; biological marker; biological product; herbaceous agent; solvent; unclassified drug; Aloe; Aloe ferox; Article; biological activity; counter current chromatography; drug isolation; herbal medicine; high performance counter current chromatography; nonhuman; phytochemistry; plant exudate; plant leaf; priority journal; reproducibility; solvent extraction; South Africa; Aloe; Aloe ferox
National Research Foundation