Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Ibadan, Ibadan, Nigeria
Ayeni, F.A., Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Ibadan, Ibadan, Nigeria; Adeniyi, B., Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Ibadan, Ibadan, Nigeria
Enterococcus faecalis FAA025 and Streptococcus equines FAA026 have been previously selected for their probiotic potentials for human application. This study was carried out to ascertain that the strains are safe for human consumption through screening for tranferable antibiotic resistant and biogenic amines genes. Antibiotic susceptibility pattern of the two bacterial strains were studied by broth microdilution. Polymerase Chain Reactions (PCR) of the isolates were tested to detect tetracycline and neomycin resistant genes. (tet W and aph 3-II). The presence of 3 biogenic amine genes (tyrosine decarboxylase, (tdc), histidine decarboxylase (hdc) and agmatine deimininase (AgDI) were screened in the 2 bacterial isolates by PCR reactions. The two bacterial strains were sensitive to most tested antibiotics. Tetracycline and neomycin resistant genes could not be detected in the two bacterial strains. The 3 biogenic amines genes were absent in Streptococcus equines FAA026 while tdc and AgDI genes were detected in Enterococcus faecalis FAA025. With reference to absence of antibiotic resistant genes and biogenic amines genes, Streptococcus equines FAA026 is safe for probiotic use while Enterococcus faecalis FAA025 has been screened out of further studies on its potential probiotic properties due to the presence of tdc and AgDI genes.
ampicillin; biogenic amine; chloramphenicol; ciprofloxacin; clindamycin; cotrimoxazole; erythromycin; fosfomycin; gentamicin; kanamycin; neomycin; nitrofurantoin; probiotic agent; streptomycin; tetracycline; AgDI gene; antibiotic resistance; antibiotic sensitivity; article; bacterial gene; bacterial strain; bacterium isolate; broth dilution; Enterococcus faecalis; gene identification; genetic screening; Hdc gene; human; microbial sensitivity test; minimum inhibitory concentration; nonhuman; product safety; risk assessment; Streptococcus equinus; tdc gene