Evaluation of antioxidant activity of Tetracarpidium conophorum (Müll. Arg) Hutch & Dalziel leaves
Oxidative Medicine and Cellular Longevity
Department of Pharmaceutical Chemistry, College of Medicine, University of Lagos, PMB 12003, Surulere, Lagos, Nigeria; Department of Pharmacognosy, College of Medicine, University of Lagos, PMB 12003, Surulere, Lagos, Nigeria
This study evaluated the antioxidant activity as well as bioflavonoid content of the methanol and ethanol-water extracts of the fresh and dried leaves of Tetracarpidium conophorum. Antioxidant activity was determined by spectrophotometric methods using DPPH free radical, nitric oxide radical inhibition and ferric reducing antioxidant power assays. In addition, total phenolics, flavonoids and proanthocyanidin content were also determined. The ethanol: water extract of the dried leaves had the highest antioxidant activity with a 50 inhibition of DPPH at a concentration of 0.017mg/mL compared to the standards, Vitamin C and Vitamin E with inhibition of 0.019 and 0.011mg/mL, respectively. This extract also showed nitric oxide radical inhibition activity comparable to that of rutin, 54.45 and 55.03 for extract and rutin, respectively, at 0.1mg/mL. Ferric reducing power was also comparable to that of ascorbic acid (281 and 287M Fe (11)/g, resp.) at a concentration of 1mg/mL. The methanol extract of both the dried and the fresh leaves had higher phenolic, flavonoids and proanthocyanidin content than the ethanol:water extract. The study reveals that T. conophorum can be an interesting source of antioxidants with their potential use in different fields namely food, cosmetics and pharmaceuticals. © 2011 O. U. Amaeze et al.
Antioxidant activities; Ascorbic acids; Dalziel; Dried leaves; Ethanol-water; Ferric reducing antioxidant power assay; Fresh leaves; Inhibition activity; Methanol extract; Proanthocyanidins; Reducing power; Spectro-photometric method; Total phenolics; Vitamin C; Vitamin-E; Water extracts; Agents; Ethanol; Flavonoids; Free radicals; Ketones; Methanol; Nitric oxide; Organic acids; Phenols; Spectrophotometers; Spectrophotometry; Solvent extraction; 1,1 diphenyl 2 picrylhydrazyl; alpha tocopherol; antioxidant; ascorbic acid; flavonoid; iron; nitric oxide; phenol derivative; plant extract; proanthocyanidin; rutoside; Tetracarpidium conophorum extract; unclassified drug; 2,2-diphenyl-1-picrylhydrazyl; antioxidant; biphenyl derivative; picric acid; antioxidant activity; article; Euphorbia; nonhuman; phytochemistry; plant leaf; Tetracarpidium conophorum; walnut; chemistry; Euphorbiaceae; plant leaf; Tetracarpidium conophorum; Antioxidants; Ascorbic Acid; Biphenyl Compounds; Euphorbiaceae; Picrates; Plant Leaves; Vitamin E