Habila N., Agbaji A.S., Ladan Z., Bello I.A., Haruna E., Dakare M.A., Atolagbe T.O.
Biochemistry Division, National Research Institute for Chemical Technology, P.M.B, 1052 Zaria, Nigeria; Department of Chemistry, Ahmadu Bello University, P.M.B, 1045 Zaria, Nigeria; Department of Biochemistry, Ahmadu Bello University, P.M.B., 1045 Zaria,
Habila, N., Biochemistry Division, National Research Institute for Chemical Technology, P.M.B, 1052 Zaria, Nigeria; Agbaji, A.S., Biochemistry Division, National Research Institute for Chemical Technology, P.M.B, 1052 Zaria, Nigeria; Ladan, Z., Biochemistry Division, National Research Institute for Chemical Technology, P.M.B, 1052 Zaria, Nigeria; Bello, I.A., Department of Chemistry, Ahmadu Bello University, P.M.B, 1045 Zaria, Nigeria; Haruna, E., Department of Biochemistry, Ahmadu Bello University, P.M.B., 1045 Zaria, Nigeria; Dakare, M.A., Biochemistry Division, National Research Institute for Chemical Technology, P.M.B, 1052 Zaria, Nigeria; Atolagbe, T.O., Department of Biochemistry, Ahmadu Bello University, P.M.B., 1045 Zaria, Nigeria
Essential oils (EOs) from Cymbopogon citratus (CC), Eucalyptus citriodora (EC), Eucalyptus camaldulensis (ED), and Citrus sinensis (CS) were obtained by hydrodistillation process. The EOs were evaluated in vitro for activity against Trypanosoma brucei brucei (Tbb) and Trypanosoma evansi (T. evansi). The EOs were found to possess antitrypanosomal activity in vitro in a dose-dependent pattern in a short period of time. The drop in number of parasite over time was achieved doses of 0.4g/ml, 0.2g/mL, and 0.1g/mL for all the EOs. The concentration of 0.4g/mL CC was more potent at 3 minutes and 2 minutes for Tbb and T. evansi, respectively. The GC-MS analysis of the EOs revealed presence of Cyclobutane (96.09) in CS, 6-octenal (77.11) in EC, Eucalyptol (75) in ED, and Citral (38.32) in CC among several other organic compounds. The results are discussed in relation to trypanosome chemotherapy. © 2010 Nathan Habila et al.