Gous N., Scott L.E., Wong E., Omar T., Venter W.D.F., Stevens W.
Department of Molecular Medicine and Haematology, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa; Wits Institute for Sexual and Reproductive Health, HIV and Related Diseases, University of the Witwatersrand, Johannesburg, South Africa; Division of Cytopathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa; National Health Laboratory Service, Johannesburg, South Africa
Gous, N., Department of Molecular Medicine and Haematology, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa; Scott, L.E., Department of Molecular Medicine and Haematology, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa; Wong, E., Wits Institute for Sexual and Reproductive Health, HIV and Related Diseases, University of the Witwatersrand, Johannesburg, South Africa; Omar, T., Division of Cytopathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa; Venter, W.D.F., Wits Institute for Sexual and Reproductive Health, HIV and Related Diseases, University of the Witwatersrand, Johannesburg, South Africa; Stevens, W., Department of Molecular Medicine and Haematology, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa, National Health Laboratory Service, Johannesburg, South Africa
The Roche LightCycler mycobacterium detection molecular assay for Mycobacterium tuberculosis, M. avium, and M. kansasii, was applied to tissue specimens. It performed well on lymph node and cerebrospinal fluid specimens and less well on lung, liver, and bone marrow core biopsy specimens, but used in conjunction with a clinical suspicion of tuberculosis, it could augment patient management. Copyright © 2012, American Society for Microbiology. All Rights Reserved.
adult; article; bacterium culture; bacterium detection; bone marrow biopsy; cerebrospinal fluid; controlled study; diagnostic accuracy; diagnostic kit; diagnostic test accuracy study; extrapulmonary tuberculosis; human; human tissue; intermethod comparison; lymph node biopsy; Mycobacterium avium; Mycobacterium kansasii; Mycobacterium tuberculosis; nonhuman; priority journal; real time polymerase chain reaction; reliability; safety; sensitivity and specificity; Adult; Aged; Aged, 80 and over; Bacteriological Techniques; Bone Marrow; Cerebrospinal Fluid; Female; Humans; Liver; Lung; Lymph Nodes; Male; Middle Aged; Molecular Diagnostic Techniques; Mycobacterium tuberculosis; Real-Time Polymerase Chain Reaction; Tuberculosis; Mycobacterium; Mycobacterium avium; Mycobacterium kansasii; Mycobacterium tuberculosis