Evaluation of existence and transmission of extended spectrum beta lactamase producing bacteria from post-delivery women to neonates at Bugando Medical Center, Mwanza-Tanzania
Department of Microbiology/Immunology, Catholic University of Health and Allied Sciences, P.O. Box 1464, Mwanza, Tanzania; Department of Biochemistry and Molecular Biology, Catholic University of Health and Allied Sciences, P.O. Box 1464, Mwanza, Tanzania; Department of Pediatrics and Child Health, Catholic University of Health and Allied Sciences, P.O. Box 1464, Mwanza, Tanzania; Department of Obstetrics and Gynaecology, Catholic University of Health and Allied Sciences, P.O. Box 1464, Mwanza, Tanzania
Background: Extended spectrum beta-lactamase producing bacteria (ESBL) are common causes of neonatal sepsis worldwide. Neonatal sepsis due to ESBL is associated with increased morbidity and mortality at Bugando Medical Centre (BMC). Due to limited information on the sources of these ESBL strains at BMC, this study was conducted to evaluate the existence, magnitude and transmission of ESBL from post-delivery women to neonates at BMC, Mwanza-Tanzania. Results: A cross-sectional study was conducted at obstetrics and neonatal wards from May to July 2013, involving post-delivery women and their neonates. Rectal swabs were collected and processed to identify the ESBL strains and their antimicrobial susceptibility patterns. Patients' data were obtained using a standardized data collection tool. We enrolled 113 women and 126 neonates with mean age of 26.5 ± 5.5 years and median gestation age [IQR] of 39 [35-40] weeks respectively. The prevalence of ESBL carriage among women and neonates were 15% (17/113) and 25.4% (32/126) respectively. The acquisition of ESBL isolates among neonates on day 1, day 3 and day 7 were 60.0% (21/35), 25.7% (9/35) and 14.3% (5/35) respectively. There was no phenotypic similarity between ESBL strains from women and their respective neonates, suggesting other sources of transmission. Neonates given antibiotics were more likely to carry ESBL than those not given [100% (32/32) versus 86% (81/94), p = 0.018]. Conclusion: The carriage rate of ESBL strains among post-delivery women and neonates at BMC is high. Our findings suggest that neonates acquire these strains from sources other than post-delivery women and more than half acquire them on the first day of life. More studies are recommended to further explore the sources of ESBL strains among neonates. © 2014 Nelson et al.; licensee BioMed Central Ltd.
antiinfective agent; beta lactamase; adult; antibiotic resistance; Bacteria; Bacterial Infections; biosynthesis; delivery; drug effects; enzymology; female; heterozygote; human; isolation and purification; microbiology; newborn; Tanzania; transmission; bacterium; Adult; Anti-Bacterial Agents; Bacteria; Bacterial Infections; beta-Lactamases; Carrier State; Delivery, Obstetric; Drug Resistance, Bacterial; Female; Humans; Infant, Newborn; Tanzania