Department of Molecular and Cell Biology, University of Cape Town, Cape Town, South Africa
Everest, G.J., Department of Molecular and Cell Biology, University of Cape Town, Cape Town, South Africa; Meyers, P.R., Department of Molecular and Cell Biology, University of Cape Town, Cape Town, South Africa
Aims: To describe three new Amycolatopsis strains and assess the antibiotic biosynthetic potential of the genus. Methods and Results: Three strains, designated S1·3T, S3·6T and SE(8)3T, belonging to the genus Amycolatopsis were isolated and found to cluster together by 16S rRNA and gyrB gene-based phylogenetic analysis. Genetic distance values, based on the gyrB gene, were calculated between the strains and their closest relatives and were all above the threshold value of 0·02 that has been proposed to distinguish Amycolatopsis type strains. DNA-DNA hybridization experiments against related type strains confirmed that strain S3·6T represents a unique genomic species. Strain S3·6T was also found to be distinct from strains S1·3T and SE(8)3T, the latter two of which were also shown to be distinct from each other. Antibiotic biosynthetic genes were identified from multiple Amycolatopsis strains, and their presence was found to be phylogenetically associated. Conclusions: The data presented in this study indicate that strains S1·3T, SE(8)3T and S3·6T belong to three novel species, for which the names Amycolatopsis circi sp. nov. (=DSM 45561T=NRRL B-24841T), Amycolatopsis equina sp. nov. (=DSM 45563T=NRRL B-24842T) and Amycolatopsis hippodromi sp. nov. (=DSM 45562T=NRRL B-24843T) are proposed. Significance and Impact of the Study: Three new species of Amycolatopsis are described, and the knowledge of the antibiotic biosynthetic potential of the genus has been extended. © 2011 The Authors. Journal of Applied Microbiology © 2011 The Society for Applied Microbiology.
ansamycin derivative; antibiotic agent; avoparcin; DNA topoisomerase (ATP hydrolysing) B; genomic DNA; RNA 16S; antibiotics; genomics; hybridization; new genus; new species; phylogenetics; Amycolatopsis alba; Amycolatopsis albidoflavus; Amycolatopsis australiensis; Amycolatopsis azurea; Amycolatopsis balhimycina; Amycolatopsis benzoatilytica; Amycolatopsis circi; Amycolatopsis coloradensis; Amycolatopsis decaplanina; Amycolatopsis echigonensis; Amycolatopsis equina; Amycolatopsis eurytherma; Amycolatopsis halophila; Amycolatopsis halotolerans; Amycolatopsis helveola; Amycolatopsis hippodromi; Amycolatopsis japonica; Amycolatopsis jejuensis; Amycolatopsis kentuckyensis; Amycolatopsis keratiniphila; Amycolatopsis lexingtonensi; Amycolatopsis lurida; Amycolatopsis marina; Amycolatopsis methanolica; Amycolatopsis minnesotensis; Amycolatopsis nigrescens; Amycolatopsis niigatensis; Amycolatopsis palatopharyngis; Amycolatopsis pigmentata; Amycolatopsis plumensis; Amycolatopsis pretoriensis; Amycolatopsis regifaucium; Amycolatopsis rifamycinica; Amycolatopsis rubida; Amycolatopsis saalfeldensis; Amycolatopsis sacchari; Amycolatopsis samaneae; Amycolatopsis taiwanensis; Amycolatopsis thailandensis; Amycolatopsis thermoflava; Amycolatopsis tolypomycina; Amycolatopsis tucumanensis; Amycolatopsis ultiminotia; Amycolatopsis vancoresmycina; Amycolatopsis xylanica; antibiotic biosynthesis; article; bacterial strain; bacterium identification; bacterium isolation; DNA hybridization; Escherichia coli; genetic analysis; Mycobacterium aurum; nocardia mediterranea; nocardia orientalis; nonhuman; nucleotide sequence; parsimony analysis; phylogenetic tree; phylogeny; Pseudonocardiaceae; Saccharopolyspora; Staphylococcus aureus; unindexed sequence; Actinomycetales; Anti-Bacterial Agents; DNA, Bacterial; Molecular Sequence Data; Phenotype; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Soil Microbiology; Actinobacteria (class); Amycolatopsis; Pseudonocardiaceae