Oluduro O.A., Idowu T.O., Aderiye B.I., Famurewa O., Omoboye O.O.
Department of Microbiology, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Nigeria; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria; College of Science, Engineering and Technology, Osun S
Oluduro, O.A., Department of Microbiology, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Nigeria; Idowu, T.O., Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria; Aderiye, B.I., Department of Microbiology, University of Ado-Ekiti, P.M.B. 5363, Ado-Ekiti, Nigeria; Famurewa, O., College of Science, Engineering and Technology, Osun State University, P.M.B. 4494, Oke-Baale, Osogbo, Nigeria, Department of Microbiology, University of Ado-Ekiti, P.M.B. 5363, Ado-Ekiti, Nigeria; Omoboye, O.O., Department of Microbiology, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Nigeria
The antibacterial activity of crude aqueous and methanolic extracts of Moringa oleifera seed on some orthopaedic wounds isolates which include Klebsiella pneumoniae, Proteus vulgaris, Providencia stuartii, Escherichia coli, Streptococcus sp., Pseudomonas fluorescens, Acinetobacter baumannii, Burkholderia cepacia, Yersinia enterocolitica, Proteus mirabilis, Serratia rubidae, Salmonella pullorum and Klebsiella oxycota was investigated. Both the crude aqueous and methanolic extracts of the seed demonstrated an appreciable inhibitory effects on the isolates with zone of growth inhibition ranging from 15 to 30.5 mm with aqueous extract and 9 to 20 mm with methanolic extract. Both extracts compared favourably with the reference standard antibiotics used. Minimum inhibitory concentration of the seed ranged from 0.875 to 5.0 μg mL -1 in aqueous extract and 0.875 to 2.5 μg mL -1 in methanolic extract. Phytochemical investigation of the methanolic extract of the seed led to the isolation and identification of new benzyl isothiocyanate and phenylmethanamine derivatives named 4-(β-D-glucopyranosyl-1-→4-α-L-rhamnopyranosyloxyl)-benzyl isothiocyanate (4) and 4-O-α-L-rhamnopyranosyloxy-N-glucopyranosyl-1-→2-fructopyranosyloxy phenylmethanamine (5) along with three known compounds, 4-hydroxyphenyl acetic acid (1), 0-methyl-4-(4'-0-acetyl-α-L-rhamnosyloxy) benzyl thiocarbamate (2) and 4-(α-L-rhamnopyranosyloxyl)-benzyl isothiocyanate (3). The structures were elucidated by extensive spectroscopic analyses which include Infra Red, Ultra Violet, Mass Spectrophotometer, ID and 2D Nuclear Magnetic Resonance spectra as well as by comparison with literature data. Both the crude aqueous and methanolic extracts displayed broad spectrum of activity as they inhibited both the Gram negative and Gram positive bacteria tested. © 2012 Academic Journals Inc.
4 (alpha rhamnopyranosyloxyl)benzyl isothiocyanic acid; 4 hydroxyphenyl acetic acid; benzyl isothiocyanate derivative; ceftriaxone; ciprofloxacin; methanol; Moringa oleifera extract; o methyl 4 (4' o acetyl alpha rhamnosyloxy)benzyl thiocarbamic acid; phenylmethanamine derivative; plant extract; unclassified drug; Acinetobacter baumannii; antibacterial activity; aqueous solution; article; bacterium isolate; Burkholderia cepacia; drug isolation; drug screening; drug structure; Escherichia coli; high performance liquid chromatography; human; infrared spectroscopy; Klebsiella oxytoca; Klebsiella pneumoniae; mass spectrometry; minimum inhibitory concentration; nuclear magnetic resonance spectroscopy; orthopedics; phytochemistry; plant seed; Proteus mirabilis; Proteus vulgaris; Providencia stuartii; Pseudomonas fluorescens; Salmonella gallinarum; Serratia; Serratia rubidae; Streptococcus; ultraviolet radiation; wound; Yersinia enterocolitica; Acinetobacter baumannii; Burkholderia cepacia; Escherichia coli; Klebsiella; Klebsiella pneumoniae; Moringa; Moringa oleifera; Negibacteria; Posibacteria; Proteus mirabilis; Proteus vulgaris; Providencia stuartii; Pseudomonas fluorescens; Salmonella enterica subsp. enterica serovar Pullorum; Serratia; Streptococcus sp.; Yersinia enterocolitica