Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, United States; Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, United States; National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, United States; Department of Laboratory Medicine, University of Washington, Seattle, WA, United States; Institute of Public Health, Makerere University, Kampala, Uganda; Department of Population, Family and Reproductive Health Sciences, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, United States; Ross Building 1159, 720 Rutland Ave., Baltimore, MD 21205, United States
Gamiel, J.L., Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, United States; Tobian, A.A.R., Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, United States; Laeyendecker, O.B., Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, United States, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, United States; Reynolds, S.J., Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, United States, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, United States; Morrow, R.A., Department of Laboratory Medicine, University of Washington, Seattle, WA, United States; Serwadda, D., Institute of Public Health, Makerere University, Kampala, Uganda; Gray, R.H., Department of Population, Family and Reproductive Health Sciences, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, United States; Quinn, T.C., Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, United States, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, United States, Ross Building 1159, 720 Rutland Ave., Baltimore, MD 21205, United States
Ugandan subjects (820) were tested by Focus HerpeSelect enzyme-linked immunosorbent assay (ELISA), Kalon herpes simplex virus type 2 ELISA, and BioKit rapid test, and the results were compared to those of Western blotting. Higher-than-standard-index cutoff values gave optimal sensitivity and specificity. Kalon ELISA was the optimal assay when an index value of 1.5 was used (sensitivity, 91.7%; specificity, 92.4%). Copyright © 2008, American Society for Microbiology. All Rights Reserved.