Lyubenova L., Bipuah H., Belford E., Michalke B., Winkler B., Schröder P.
Department of Environmental Seienees, Researeh Unit Mierobe-Plant Interaetions, German Researeh Center for Environmental Health, Helmholtz Zentrum Münehen, Ingolstädter Landstrasse 1, Neuherberg, Germany; Department of Theoretieal and Applied Biology, College of Seienee, Kwame Nkrumah University of Seienee and Teehnology, Kumasi, Ghana; German Researeh Center for Environmental Health, Institute of Eeologieal Chemistry, Helmholtz Zentrum Münehen, Ingolstädter Landstrasse 1, Neuherberg, Germany; Department of Environmental Seienees, Researeh Unit Environmental Simulation, German Researeh Center for Environmental Health,Helmholtz Zentrum Münehen, Ingolstädter Landstrasse 1, Neuherberg, Germany
Lyubenova, L., Department of Environmental Seienees, Researeh Unit Mierobe-Plant Interaetions, German Researeh Center for Environmental Health, Helmholtz Zentrum Münehen, Ingolstädter Landstrasse 1, Neuherberg, Germany; Bipuah, H., Department of Theoretieal and Applied Biology, College of Seienee, Kwame Nkrumah University of Seienee and Teehnology, Kumasi, Ghana; Belford, E., Department of Theoretieal and Applied Biology, College of Seienee, Kwame Nkrumah University of Seienee and Teehnology, Kumasi, Ghana; Michalke, B., German Researeh Center for Environmental Health, Institute of Eeologieal Chemistry, Helmholtz Zentrum Münehen, Ingolstädter Landstrasse 1, Neuherberg, Germany; Winkler, B., Department of Environmental Seienees, Researeh Unit Environmental Simulation, German Researeh Center for Environmental Health,Helmholtz Zentrum Münehen, Ingolstädter Landstrasse 1, Neuherberg, Germany; Schröder, P., Department of Environmental Seienees, Researeh Unit Mierobe-Plant Interaetions, German Researeh Center for Environmental Health, Helmholtz Zentrum Münehen, Ingolstädter Landstrasse 1, Neuherberg, Germany
The present study focused on cupric sulphate and cupric nitrate uptake in Typha latifolia and the impact of these copper species on the plant's detoxification capacity. When the plants were exposed to 10, 50 and 100 (oMcupric sulphate or cupric nitrate, copper accumulation in T. latifolia roots and shoots increased with rising concentration of the salts. Shoot to root ratios differed significantly depending on the form of copper supplementation, e.g. if it was added as cupric (II) sulphate or cupric (II) nitrate. After incubation with 100 \iM of cupric sulphate, up to 450 mg Cu/kg fresh weight (FW) was accumulated, whereas the same concentration of cupric nitrate resulted in accumulation of 580 mg/kg FW. Furthermore, significant differences in the activity of some antioxidative enzymes in Typha roots compared to the shoots, which are essential in the plant's reaction to cope with metal stress, were observed. The activity of peroxidase (POX) in roots was increased at intermediate concentrations (10 and 50 |oM) of CuSO<inf>4</inf>, whereas it was inhibited at the same Cu(NO<inf>3</inf>)<inf>2</inf> concentrations. Ascorbate peroxidase (APOX) and dehydroascorbate reductase (DHAR) increased their enzyme activity intensely, which may be an indication for copper toxicity in T latifolia plants. Besides, fluorodifen conjugation by glutathione S-transferases (GSTs) was increased up to. © Springer-Verlag Berlin Heidelberg 2014.