Biochemical evaluation of leachate-contaminated groundwater on the kidney of Albino rats
Experimental and Toxicologic Pathology
Department of Environmental Sciences, Federal University of Petroleum Resources, PMB 1221, Effurun, Delta State, Nigeria; Department of Biochemistry, University of Ilorin, Ilorin, Nigeria
The effect of leachate-contaminated groundwater on the cells of the kidney was evaluated. Serum Na+ concentration of control rats was observed to be 120±1.0nmol/l while that of rat placed on simulated leachate was 180±4.0nmol/l. Serum K+, urea and creatinine concentrations of rats placed on simulated leachate and leachate-contaminated groundwater were significantly higher(p<0.05) than those of control rats. The activity of Alkaline phosphatase (ALP) of the kidney and serum, respectively, observed for the control rats were (237±3.70 and 0.37±0.01)nmol/min/mg protein while (116±4.20 and 3.17±0.20)nmol/min/mg protein was the ALP activity of kidney and serum, respectively, observed for the rats placed on simulated leachate. Histological examination of the kidney of the control rats showed no visible lesion while that of rats placed on simulated leachate showed extensive necrosis of muscle fibres and cellular infiltration by macrophages. It is viewed that leachate-contaminated groundwater may damage kidney cells and impair renal function. © 2009 Elsevier GmbH.
alkaline phosphatase; creatinine; ground water; potassium ion; sodium ion; urea; alkaline phosphatase blood level; animal experiment; animal tissue; article; biochemistry; bleeding; cell damage; cell infiltration; controlled study; creatinine blood level; enzyme activity; histopathology; kidney cell; kidney dysfunction; kidney parenchyma; leaching; macrophage; muscle necrosis; nonhuman; potassium blood level; rat; simulation; sodium blood level; urea blood level; water contamination; Animals; Creatinine; Kidney; Kidney Function Tests; Rats; Urea; Water Pollutants, Chemical; Rattus